Biodegradation of TCE by mixed cultures

Naresh Singhal, Peter R. Jaffe, Walter Maier

Research output: Contribution to journalConference articlepeer-review


Batch experiments were performed to evaluate the effect of different carbon sources (methanol and sodium formate) on the degradation of TCE by mixed cultures of methanogens and fermenters. Vials containing growth media, fermentative and methanogenic bacteria, methanol, sodium formate, and trichloroethylene (TCE) were incubated at 25°C. The total theoretical organic carbon (ThOC) of sodium formate plus methanol was always 3,000 mg/l. The initial TCE concentration in the vials was 0.034 mg/l. The contents of the vials were analyzed for pH, Eh, CH4, CO2, TCE and TCE daughter products after a 3 week incubation period. Results show that pH increased monotonically from 6.5 (for 100% methanol) to 8.5 (for 100% sodium formate), whereas Eh decreased from -440 to -550 mV, respectively. Additional study results are discussed.

Original languageEnglish (US)
Pages (from-to)915-916
Number of pages2
JournalNational Conference on Environmental Engineering
StatePublished - 1990
EventProceedings of the 1990 Specialty Conference - Arlington, VA, USA
Duration: Jul 8 1990Jul 11 1990

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • General Engineering


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