bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring

Vítor Trovisco; Katsiaryna Belaya; Dmitry Nashchekin; Uwe Irion; George Sirinakis; Richard Butler; Jack J. Lee; Elizabeth R. Gavis; Daniel St Johnston

doi:10.7554/eLife.17537

bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring

Vítor Trovisco, Katsiaryna Belaya, Dmitry Nashchekin, Uwe Irion, George Sirinakis, Richard Butler, Jack J. Lee, Elizabeth R. Gavis, Daniel St Johnston

Molecular Biology

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29 Scopus citations

Abstract

bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110–120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.

Original language	English (US)
Article number	e17537
Journal	eLife
Volume	5
Issue number	OCTOBER2016
DOIs	https://doi.org/10.7554/eLife.17537
State	Published - Oct 28 2016

All Science Journal Classification (ASJC) codes

General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience

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10.7554/eLife.17537

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title = "bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring",

abstract = "bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110–120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.",

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T1 - bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring

AU - Trovisco, Vítor

AU - Belaya, Katsiaryna

AU - Nashchekin, Dmitry

AU - Irion, Uwe

AU - Sirinakis, George

AU - Butler, Richard

AU - Lee, Jack J.

AU - Gavis, Elizabeth R.

AU - St Johnston, Daniel

PY - 2016/10/28

Y1 - 2016/10/28

N2 - bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110–120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.

AB - bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110–120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.

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bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring

Abstract

All Science Journal Classification (ASJC) codes

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