bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring

Vítor Trovisco, Katsiaryna Belaya, Dmitry Nashchekin, Uwe Irion, George Sirinakis, Richard Butler, Jack J. Lee, Elizabeth R. Gavis, Daniel St Johnston

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110–120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.

Original languageEnglish (US)
Article numbere17537
JournaleLife
Volume5
Issue numberOCTOBER2016
DOIs
StatePublished - Oct 28 2016

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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