Bacterial chromosome extraction and isolation

Christelle Prinz; Jonas O. Tegenfeldt; Robert H. Austin; Edward C. Cox; James C. Sturm

doi:10.1039/b208010a

Bacterial chromosome extraction and isolation

Christelle Prinz, Jonas O. Tegenfeldt, Robert H. Austin, Edward C. Cox, James C. Sturm

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80 Scopus citations

Abstract

We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.

Original language	English (US)
Pages (from-to)	207-212
Number of pages	6
Journal	Lab on a Chip
Volume	2
Issue number	4
DOIs	https://doi.org/10.1039/b208010a
State	Published - Nov 1 2002

All Science Journal Classification (ASJC) codes

General Chemistry
Bioengineering
Biochemistry
Biomedical Engineering

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10.1039/b208010a

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title = "Bacterial chromosome extraction and isolation",

abstract = "We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.",

author = "Christelle Prinz and Tegenfeldt, {Jonas O.} and Austin, {Robert H.} and Cox, {Edward C.} and Sturm, {James C.}",

year = "2002",

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doi = "10.1039/b208010a",

language = "English (US)",

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journal = "Lab on a Chip",

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publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Bacterial chromosome extraction and isolation

AU - Prinz, Christelle

AU - Tegenfeldt, Jonas O.

AU - Austin, Robert H.

AU - Cox, Edward C.

AU - Sturm, James C.

PY - 2002/11/1

Y1 - 2002/11/1

N2 - We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.

AB - We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.

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U2 - 10.1039/b208010a

DO - 10.1039/b208010a

M3 - Article

C2 - 15100812

AN - SCOPUS:0141989836

SN - 1473-0197

VL - 2

SP - 207

EP - 212

JO - Lab on a Chip

JF - Lab on a Chip

IS - 4

ER -

Bacterial chromosome extraction and isolation

Abstract

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