Bacterial chromosome extraction and isolation

Christelle Prinz, Jonas O. Tegenfeldt, Robert H. Austin, Edward C. Cox, James C. Sturm

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.

Original languageEnglish (US)
Pages (from-to)207-212
Number of pages6
JournalLab on a Chip
Volume2
Issue number4
DOIs
StatePublished - Nov 1 2002

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Bacterial chromosome extraction and isolation'. Together they form a unique fingerprint.

  • Cite this