Auxiliary-mediated site-specific peptide ubiquitylation

Champak Chatterjee, Robert K. McGinty, Jean Philippe Pellois, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

(Chemical Equation Presented) Lighting up ubiquitylation: A photolytically removable ligation auxiliary (Aux) was employed for the site-specific attachment of ubiquitin (Ub) to one of three lysine residues in a C-terminal peptide fragment of histone H2B (cH2B). Following photolysis, the ligation product was a viable substrate for the ubiquitin C-terminal hydrolase UCH-L3.

Original languageEnglish (US)
Pages (from-to)2814-2818
Number of pages5
JournalAngewandte Chemie - International Edition
Volume46
Issue number16
DOIs
StatePublished - 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Keywords

  • Ligation auxiliaries
  • Peptides
  • Semisynthesis
  • Ubiquitylation

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