TY - JOUR
T1 - Auditory activity is diverse and widespread throughout the central brain of Drosophila
AU - Pacheco, Diego A.
AU - Thiberge, Stephan Y.
AU - Pnevmatikakis, Eftychios
AU - Murthy, Mala
N1 - Funding Information:
We thank J. Clemens for assistance on auditory stimuli delivery and linear modeling of calcium responses, as well as helpful discussions on auditory coding; A. Giovannucci for help with ROI segmentation of volumetric calcium signals and guidance with CaImAn toolbox usage; G. Jefferis and T. Rohlfing for help using the image registration toolbox CMTK and the neuroanatomy toolbox natverse; B. Cowley for help with linear modeling of calcium responses in behaving flies; A. Calhoun for help with analysis of the hemibrain connectome dataset; and T. Pereira for assistance with the CPD algorithm. We also thank G. Jefferis, S. Ahmed, C. Baker and J. Clemens for comments on the manuscript. D.A.P. was supported in part by a NSF Physics Frontier Center grant, and M.M. was supported by a NIH NINDS New Innovator award, NIH BRAIN Initiative R01s NS104899 and NS110060, and a Howard Hughes Medical Institute Faculty Scholar award.
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2021/1
Y1 - 2021/1
N2 - Sensory pathways are typically studied by starting at receptor neurons and following postsynaptic neurons into the brain. However, this leads to a bias in analyses of activity toward the earliest layers of processing. Here, we present new methods for volumetric neural imaging with precise across-brain registration to characterize auditory activity throughout the entire central brain of Drosophila and make comparisons across trials, individuals and sexes. We discover that auditory activity is present in most central brain regions and in neurons responsive to other modalities. Auditory responses are temporally diverse, but the majority of activity is tuned to courtship song features. Auditory responses are stereotyped across trials and animals in early mechanosensory regions, becoming more variable at higher layers of the putative pathway, and this variability is largely independent of ongoing movements. This study highlights the power of using an unbiased, brain-wide approach for mapping the functional organization of sensory activity.
AB - Sensory pathways are typically studied by starting at receptor neurons and following postsynaptic neurons into the brain. However, this leads to a bias in analyses of activity toward the earliest layers of processing. Here, we present new methods for volumetric neural imaging with precise across-brain registration to characterize auditory activity throughout the entire central brain of Drosophila and make comparisons across trials, individuals and sexes. We discover that auditory activity is present in most central brain regions and in neurons responsive to other modalities. Auditory responses are temporally diverse, but the majority of activity is tuned to courtship song features. Auditory responses are stereotyped across trials and animals in early mechanosensory regions, becoming more variable at higher layers of the putative pathway, and this variability is largely independent of ongoing movements. This study highlights the power of using an unbiased, brain-wide approach for mapping the functional organization of sensory activity.
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U2 - 10.1038/s41593-020-00743-y
DO - 10.1038/s41593-020-00743-y
M3 - Article
C2 - 33230320
AN - SCOPUS:85096452415
VL - 24
SP - 93
EP - 104
JO - Nature Neuroscience
JF - Nature Neuroscience
SN - 1097-6256
IS - 1
ER -