@article{bcd0e069dfd94d4199afb59976f2d336,
title = "Asymmetric allosteric activation of the symmetric ArgR hexamer",
abstract = "Hexameric arginine repressor, ArgR, bound to l-arginine serves both as the master transcriptional repressor/activator at diverse regulons in a wide range of bacteria and as a required cofactor for resolution of ColE1 plasmid multimers. Multifunctional ArgR is thus unusual in possessing features of specific gene regulators, global regulators, and non-specific gene organizers; its closest functional analog is probably CAP, the cyclic AMP receptor/activator protein. Isothermal titration calorimetry, surface plasmon resonance, and proteolysis indicate that binding of a single l-arginine residue per ArgR hexamer triggers a global conformational change and resets the affinities of the remaining five sites, making them 100-fold weaker. The analysis suggests a novel thermodynamic signature for this mechanism of activation.",
keywords = "c value, cooperativity, global analysis, ligand occupancy",
author = "Lihua Jin and Xue, {Wei Feng} and Fukayama, {June Wong} and Jaclyn Yetter and Michael Pickering and Jannette Carey",
note = "Funding Information: We are grateful for the support of the National Science Foundation (DBI-CCLI AI01-26642 to L.J. and MCB01-36094 to J.C.) and DePaul University College of Liberal Arts and Sciences (2003 faculty summer grant research). We thank Sara Linse, Lund University, and P{\"a}r S{\"a}fsten, Biacore AB, Uppsala, for generously providing access to Biacore-3000 instruments; Jaymie DeWitt, Biacore, Inc. USA, for conducting pilot Biacore experiments; and Danuta Szwajkajzer for her early contributions to analysis of Biacore data. We thank David Remeta and Kenneth Breslauer, Rutgers University, for their generosity in conducting preliminary ITC experiments using their Microcal calorimeter. Undergraduate students Nicole Follmer and Huan Zheng, Princeton University, and Alissa Agnello, Wellesley College, produced many of the preparations of ArgR and ArgRC proteins used in this work. Rita Grandori, Fred Hughson, and Stefano Ricagno provided insightful comments on the manuscript.",
year = "2005",
month = feb,
day = "11",
doi = "10.1016/j.jmb.2004.11.031",
language = "English (US)",
volume = "346",
pages = "43--56",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "1",
}