@article{fec48a8d3d7d4172a7c7306ae1459deb,
title = "Assessing the Viability of Recovery of Hydroxycinnamic Acids from Lignocellulosic Biorefinery Alkaline Pretreatment Waste Streams",
abstract = "The hydroxycinnamic acids p-coumaric acid (pCA) and ferulic acid (FA) add diversity to the portfolio of products produced by using grass-fed lignocellulosic biorefineries. The level of lignin-bound pCA in Zea mays was modified by the alteration of p-coumaroyl-CoA monolignol transferase expression. The biomass was processed in a lab-scale alkaline-pretreatment biorefinery process and the data were used for a baseline technoeconomic analysis to determine where to direct future research efforts to couple plant design to biomass utilization processes. It is concluded that future plant engineering efforts should focus on strategies that ramp up accumulation of one type of hydroxycinnamate (pCA or FA) predominantly and suppress that of the other. Technoeconomic analysis indicates that target extraction titers of one hydroxycinnamic acid need to be >50 g kg−1 biomass, at least five times higher than observed titers for the impure pCA/FA product mixture from wild-type maize. The technical challenge for process engineers is to develop a viable process that requires more than 80 % reduction of the isolation costs.",
keywords = "acids, biomass, industrial chemistry, protein engineering, synthesis design",
author = "Karlen, {Steven D.} and Peyman Fasahati and Mona Mazaheri and Jose Serate and Smith, {Rebecca A.} and Sirisha Sirobhushanam and Mingjie Chen and Tymokhin, {Vitaliy I.} and Cass, {Cynthia L.} and Sarah Liu and Dharshana Padmakshan and Dan Xie and Yaoping Zhang and McGee, {Mick A.} and Russell, {Jason D.} and Coon, {Joshua J.} and Kaeppler, {Heidi F.} and {de Leon}, Natalia and Maravelias, {Christos T.} and Runge, {Troy M.} and Kaeppler, {Shawn M.} and Sedbrook, {John C.} and John Ralph",
note = "Funding Information: Funding : This project was funded by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science DE ‐ FC02 ‐ 07ER64494 and DE‐SC0018409). Funding Information: General: We thank Jonas Rodriguez and Marlies Heckwolf for assistance in the field trials, RJ Heim, Evan Rocco, and Justin Mobley for assistance in harvesting and preparing the sample, Cliff Foster the GLBRC Biomass Analytics Facility at Michigan State University for assistance in screening the individual plants, and Hoon Kim for useful discussions. Funding: This project was funded by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science DE-FC02-07ER64494 and DE-SC0018409). Data and Materials availability: The DNA sequences for the ZmPMT (pCAT, Protein ID GRMZM2G028104) and BdPMT (Protein ID Bradi2g36910) genes can be found in GenBank. Publisher Copyright: {\textcopyright} 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.",
year = "2020",
month = apr,
day = "21",
doi = "10.1002/cssc.201903345",
language = "English (US)",
volume = "13",
pages = "2012--2024",
journal = "ChemSusChem",
issn = "1864-5631",
publisher = "Wiley-VCH Verlag",
number = "8",
}