Analysis of inactivation of AcMNPV under various conditions by the ELVA method

Takeshi Gotoh; Nozomi Ando; Ken Ichi Kikuchi

doi:10.1271/bbb.80105

Analysis of inactivation of AcMNPV under various conditions by the ELVA method

Takeshi Gotoh, Nozomi Ando, Ken Ichi Kikuchi

Chemistry

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Inactivation of baculoviruses was examined under various conditions by ELVA, which enzymatically labels the virus envelope with radioisotopes and detects the labeled viruses by host cell-specific binding. When baculoviruses were incubated in a 50-ml culture tube, they rapidly lost infective ability by more than 75% of the initial titer in 2 h even at an insect cell cultivation temperature of 27 °C. Altering pH from neutral significantly decreased the virus titer. Repetition of freezing and thawing of baculovirus solutions decreased the virus titer, but the addition of DMSO and glycerol prevented inactivation to some extent.

Original language	English (US)
Pages (from-to)	1973-1976
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	7
DOIs	https://doi.org/10.1271/bbb.80105
State	Published - 2008

All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology
Analytical Chemistry
Molecular Biology
Biochemistry
Biotechnology
Organic Chemistry

Keywords

Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
Baculoviruses
Envelope-labeled virus assay (ELVA)
Inactivation
Stability

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10.1271/bbb.80105

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title = "Analysis of inactivation of AcMNPV under various conditions by the ELVA method",

abstract = "Inactivation of baculoviruses was examined under various conditions by ELVA, which enzymatically labels the virus envelope with radioisotopes and detects the labeled viruses by host cell-specific binding. When baculoviruses were incubated in a 50-ml culture tube, they rapidly lost infective ability by more than 75% of the initial titer in 2 h even at an insect cell cultivation temperature of 27 °C. Altering pH from neutral significantly decreased the virus titer. Repetition of freezing and thawing of baculovirus solutions decreased the virus titer, but the addition of DMSO and glycerol prevented inactivation to some extent.",

keywords = "Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Baculoviruses, Envelope-labeled virus assay (ELVA), Inactivation, Stability",

author = "Takeshi Gotoh and Nozomi Ando and Kikuchi, {Ken Ichi}",

note = "Funding Information: This work was supported in part by a Grant-in Aid for Scientific Research (no. 16560680) from the Japan Society for the Promotion of Science (JSPS).",

year = "2008",

doi = "10.1271/bbb.80105",

language = "English (US)",

volume = "72",

pages = "1973--1976",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

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TY - JOUR

T1 - Analysis of inactivation of AcMNPV under various conditions by the ELVA method

AU - Gotoh, Takeshi

AU - Ando, Nozomi

AU - Kikuchi, Ken Ichi

N1 - Funding Information: This work was supported in part by a Grant-in Aid for Scientific Research (no. 16560680) from the Japan Society for the Promotion of Science (JSPS).

PY - 2008

Y1 - 2008

N2 - Inactivation of baculoviruses was examined under various conditions by ELVA, which enzymatically labels the virus envelope with radioisotopes and detects the labeled viruses by host cell-specific binding. When baculoviruses were incubated in a 50-ml culture tube, they rapidly lost infective ability by more than 75% of the initial titer in 2 h even at an insect cell cultivation temperature of 27 °C. Altering pH from neutral significantly decreased the virus titer. Repetition of freezing and thawing of baculovirus solutions decreased the virus titer, but the addition of DMSO and glycerol prevented inactivation to some extent.

AB - Inactivation of baculoviruses was examined under various conditions by ELVA, which enzymatically labels the virus envelope with radioisotopes and detects the labeled viruses by host cell-specific binding. When baculoviruses were incubated in a 50-ml culture tube, they rapidly lost infective ability by more than 75% of the initial titer in 2 h even at an insect cell cultivation temperature of 27 °C. Altering pH from neutral significantly decreased the virus titer. Repetition of freezing and thawing of baculovirus solutions decreased the virus titer, but the addition of DMSO and glycerol prevented inactivation to some extent.

KW - Autographa californica multiple nucleopolyhedrovirus (AcMNPV)

KW - Baculoviruses

KW - Envelope-labeled virus assay (ELVA)

KW - Inactivation

KW - Stability

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SN - 0916-8451

VL - 72

SP - 1973

EP - 1976

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 7

ER -

Analysis of inactivation of AcMNPV under various conditions by the ELVA method

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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