Abstract
Authors describe in this paper the isolation and characterization of a class of mutations, designated puq, that allow phage λ to grow better under conditions that limit the synthesis of the phage Q gene product. These mutations were located between phage genes P and Q, a region of the λ chromosome containing two gene N-independent mutations, nin5 and byp, that we also show to be puq mutations. Whereas the puq-3 and puq-16 mutations probably map under the nin5 deletion, the byp mutation maps between this deletion and the Qλ-Qphi80 crossover point. These mutations likely act by increasing the synthesis of the Q gene product. We demonstrate that the clear-plaque phenotype and reduced lysogenization frequency of byp mutants depend on increased Q gene activity. The significance of these results in understanding how transcription proceeds through the P-Q region of the λ genome is discussed.
Original language | English (US) |
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Pages (from-to) | 1-13 |
Number of pages | 13 |
Journal | Journal of virology |
Volume | 30 |
Issue number | 1 |
DOIs | |
State | Published - 1979 |
All Science Journal Classification (ASJC) codes
- Insect Science
- Virology
- Microbiology
- Immunology