Analysis of cell migration using caenorhabditis elegans as a model system

Ming Ching Wong, Maria Martynovsky, Jean E. Schwarzbauer

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

The nematode Caenorhabditis elegans is an excellent model system in which to study long-distance cell migration in vivo. This chapter describes methods used to study a subset of migratory cells in the hermaphrodite nematode, the distal tip cells. These methods take advantage of the organism's transparent body and the expression of green fluorescent protein to observe cell migration and behavior. Additionally, the availability of nematode mutants and gene knockdown techniques that affect cell migration allow the analysis and comparison of wild-type and aberrant migratory paths. Methods for nematode growth and maintenance, strain acquisition, observation and live imaging, gene knockdown, and analysis of cell migration defects are covered.

Original languageEnglish (US)
Title of host publicationCell Migration
Subtitle of host publicationDevelopmental Methods and Protocols
EditorsClaire M. Wells, Maddy Parsons
Pages233-247
Number of pages15
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume769
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • C. elegans
  • Cell migration
  • Distal tip cells
  • Green fluorescent protein
  • Live imaging
  • Mutants
  • RNAi

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