TY - JOUR
T1 - Anaerobic membrane gas extraction facilitates thermophilic hydrogen production from Clostridium thermocellum
AU - Singer, Scott
AU - Magnusson, Lauren
AU - Hou, Dianxun
AU - Lo, Jonathan
AU - Maness, Pin Ching
AU - Ren, Zhiyong Jason
N1 - Funding Information:
The authors acknowledged the financial support provided by the National Science Foundation (CBET 1510682 to S. S., D. H., and Z. J. R.) as well as the Department of Energy (DOE) Energy Efficiency and Renewable Energy Fuel Cell Technologies Office (to L. M., J. L., and P. C. M.). This work was authorized in part by Alliance for Sustainable Energy, LLC, the manager and operator of the National Renewable Energy Laboratory for the U.S. DOE under Contract No. DE-AC36-08GO28308.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2018/11
Y1 - 2018/11
N2 - Clostridium thermocellum is among the most efficient bacteria to convert cellulosic biomass into H2 during dark fermentation. However, despite great progress the H2 yield and rate are still not satisfactory for large scale applications. The purpose of this study was to evaluate whether in situ gas extraction using membrane bioreactors would increase H2 production from Clostridium thermocellum when compared to a conventional anaerobic fermentation setup in thermophilic conditions. C. thermocellum DSM 1313, a cellulotyic, thermophilic bacterium was grown on cellobiose and Avicel in an anaerobic-fermenter (AF) and an anaerobic-membrane-bioreactor (AnMBR). Compared to the AF, the AnMBR increased cumulative H2 production by 63%, from 25.8 to 42.1 mmols, increased the max H2 production rate by 24%, from 3.4 to 4.2 mmol h−1, and increased yield by 58%, from 0.43 to 0.68 mmol H2 per mmol hexose, on cellobiose. Likewise, on Avicel, the AnMBR increased cumulative H2 production by 59%, from 46.8 to 74.6 mmols, increased the max H2 rate by 87%, from 3.1 to 5.8 mmol h−1, and increased the yield by 59%, from 0.76 to 1.21 mmol H2 per mmol hexose. These results show that anaerobic membrane gas extraction can be an effective approach to increasing both rate and yield of fermentative H2 production.
AB - Clostridium thermocellum is among the most efficient bacteria to convert cellulosic biomass into H2 during dark fermentation. However, despite great progress the H2 yield and rate are still not satisfactory for large scale applications. The purpose of this study was to evaluate whether in situ gas extraction using membrane bioreactors would increase H2 production from Clostridium thermocellum when compared to a conventional anaerobic fermentation setup in thermophilic conditions. C. thermocellum DSM 1313, a cellulotyic, thermophilic bacterium was grown on cellobiose and Avicel in an anaerobic-fermenter (AF) and an anaerobic-membrane-bioreactor (AnMBR). Compared to the AF, the AnMBR increased cumulative H2 production by 63%, from 25.8 to 42.1 mmols, increased the max H2 production rate by 24%, from 3.4 to 4.2 mmol h−1, and increased yield by 58%, from 0.43 to 0.68 mmol H2 per mmol hexose, on cellobiose. Likewise, on Avicel, the AnMBR increased cumulative H2 production by 59%, from 46.8 to 74.6 mmols, increased the max H2 rate by 87%, from 3.1 to 5.8 mmol h−1, and increased the yield by 59%, from 0.76 to 1.21 mmol H2 per mmol hexose. These results show that anaerobic membrane gas extraction can be an effective approach to increasing both rate and yield of fermentative H2 production.
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U2 - 10.1039/c8ew00289d
DO - 10.1039/c8ew00289d
M3 - Article
AN - SCOPUS:85052064017
SN - 2053-1400
VL - 4
SP - 1771
EP - 1782
JO - Environmental Science: Water Research and Technology
JF - Environmental Science: Water Research and Technology
IS - 11
ER -