Abstract
This paper presents the results of an experimental study of the initial cell spreading and adhesion on longitudinally- and transversally-oriented micro-grooves produced by the laser irradiation of laser grooved Ti-6Al-4V surfaces. The initial spreading and orientations of human osteosarcoma (HOS) cells were observed and quantified after 15-min, 1-hour, 4-hour and 24-hour cell culture periods. Immuno-fluorescence staining of adhesion proteins (actin and vinculin) was then used to study the spreading and adhesion of HOS cells in 1 hour and 4 hour culture experiments. The initial cell adhesion was also quantified using enzymatic detachment tests. The results showed that cell spreading and adhesion were enhanced by longitudinally- and transversally-oriented micro-grooves. The effects, which increase with time, were not remarkable after 1 hour, but obvious after 4 hours. Contact guidance was found to promote cell adhesion due to the increase in interactions between the focal adhesions and the patterned extra-cellular matrix (ECM) proteins on the laser micro-grooved surfaces.
Original language | English (US) |
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Pages (from-to) | 1442-1452 |
Number of pages | 11 |
Journal | Materials Science and Engineering C |
Volume | 29 |
Issue number | 4 |
DOIs | |
State | Published - May 5 2009 |
All Science Journal Classification (ASJC) codes
- General Medicine
Keywords
- Contact guidance
- Initial cell spreading and adhesion
- Laser processing
- Microgrooves
- Titanium texturing