An epigenetic aging clock for dogs and wolves

Michael J. Thompson; Bridgett Marie vonHoldt; Steve Horvath; Matteo Pellegrini

doi:10.18632/aging.101211

An epigenetic aging clock for dogs and wolves

Michael J. Thompson, Bridgett Marie vonHoldt, Steve Horvath, Matteo Pellegrini

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

Several articles describe highly accurate age estimation methods based on human DNA-methylation data. It is not yet known whether similar epigenetic aging clocks can be developed based on blood methylation data from canids. Using Reduced Representation Bisulfite Sequencing, we assessed blood DNA-methylation data from 46 domesticated dogs (Canis familiaris) and 62 wild gray wolves (C. lupus). By regressing chronological dog age on the resulting CpGs, we defined highly accurate multivariate age estimators for dogs (based on 41 CpGs), wolves (67 CpGs), and both combined (115 CpGs). Age related DNA methylation changes in canids implicate similar gene ontology categories as those observed in humans suggesting an evolutionarily conserved mechanism underlying age-related DNA methylation in mammals.

Original language	English (US)
Pages (from-to)	1055-1068
Number of pages	14
Journal	Aging
Volume	9
Issue number	3
DOIs	https://doi.org/10.18632/aging.101211
State	Published - 2017

All Science Journal Classification (ASJC) codes

Aging
Cell Biology

Keywords

Biological age
Canine
DNA methylation
Dog
Epigenetic clock
Wolf

Access to Document

10.18632/aging.101211

Cite this

@article{0fc1e1df24904b308b1b508213feb8da,

title = "An epigenetic aging clock for dogs and wolves",

abstract = "Several articles describe highly accurate age estimation methods based on human DNA-methylation data. It is not yet known whether similar epigenetic aging clocks can be developed based on blood methylation data from canids. Using Reduced Representation Bisulfite Sequencing, we assessed blood DNA-methylation data from 46 domesticated dogs (Canis familiaris) and 62 wild gray wolves (C. lupus). By regressing chronological dog age on the resulting CpGs, we defined highly accurate multivariate age estimators for dogs (based on 41 CpGs), wolves (67 CpGs), and both combined (115 CpGs). Age related DNA methylation changes in canids implicate similar gene ontology categories as those observed in humans suggesting an evolutionarily conserved mechanism underlying age-related DNA methylation in mammals.",

keywords = "Biological age, Canine, DNA methylation, Dog, Epigenetic clock, Wolf",

author = "Thompson, {Michael J.} and vonHoldt, {Bridgett Marie} and Steve Horvath and Matteo Pellegrini",

note = "Funding Information: SH, MP, and MT were supported by 1R21AG049400-01A1. SH was funded by NIH/NIA U34AG051425-01. MJT acknowledges support from a QCB Collaboratory Postdoctoral Fellowship, and the QCB Collaboratory community directed by Matteo Pellegrini. BvH acknowledges support from National Science Foundation (Grant Numbers: DEB-0613730, DEB-1245373, DMS-1264153), Yellowstone National Park, the Yellowstone Park Foundation, the Intramural Program of the National Human Genome Research Institute, AKC OAK (Grant Number: 1822), and the NIH (Grant Numbers: T32 HG002536, GM053275).",

year = "2017",

doi = "10.18632/aging.101211",

language = "English (US)",

volume = "9",

pages = "1055--1068",

journal = "Aging",

issn = "1945-4589",

publisher = "US Administration on Aging",

number = "3",

}

TY - JOUR

T1 - An epigenetic aging clock for dogs and wolves

AU - Thompson, Michael J.

AU - vonHoldt, Bridgett Marie

AU - Horvath, Steve

AU - Pellegrini, Matteo

N1 - Funding Information: SH, MP, and MT were supported by 1R21AG049400-01A1. SH was funded by NIH/NIA U34AG051425-01. MJT acknowledges support from a QCB Collaboratory Postdoctoral Fellowship, and the QCB Collaboratory community directed by Matteo Pellegrini. BvH acknowledges support from National Science Foundation (Grant Numbers: DEB-0613730, DEB-1245373, DMS-1264153), Yellowstone National Park, the Yellowstone Park Foundation, the Intramural Program of the National Human Genome Research Institute, AKC OAK (Grant Number: 1822), and the NIH (Grant Numbers: T32 HG002536, GM053275).

PY - 2017

Y1 - 2017

N2 - Several articles describe highly accurate age estimation methods based on human DNA-methylation data. It is not yet known whether similar epigenetic aging clocks can be developed based on blood methylation data from canids. Using Reduced Representation Bisulfite Sequencing, we assessed blood DNA-methylation data from 46 domesticated dogs (Canis familiaris) and 62 wild gray wolves (C. lupus). By regressing chronological dog age on the resulting CpGs, we defined highly accurate multivariate age estimators for dogs (based on 41 CpGs), wolves (67 CpGs), and both combined (115 CpGs). Age related DNA methylation changes in canids implicate similar gene ontology categories as those observed in humans suggesting an evolutionarily conserved mechanism underlying age-related DNA methylation in mammals.

AB - Several articles describe highly accurate age estimation methods based on human DNA-methylation data. It is not yet known whether similar epigenetic aging clocks can be developed based on blood methylation data from canids. Using Reduced Representation Bisulfite Sequencing, we assessed blood DNA-methylation data from 46 domesticated dogs (Canis familiaris) and 62 wild gray wolves (C. lupus). By regressing chronological dog age on the resulting CpGs, we defined highly accurate multivariate age estimators for dogs (based on 41 CpGs), wolves (67 CpGs), and both combined (115 CpGs). Age related DNA methylation changes in canids implicate similar gene ontology categories as those observed in humans suggesting an evolutionarily conserved mechanism underlying age-related DNA methylation in mammals.

KW - Biological age

KW - Canine

KW - DNA methylation

KW - Dog

KW - Epigenetic clock

KW - Wolf

UR - http://www.scopus.com/inward/record.url?scp=85016635434&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016635434&partnerID=8YFLogxK

U2 - 10.18632/aging.101211

DO - 10.18632/aging.101211

M3 - Article

C2 - 28373601

AN - SCOPUS:85016635434

SN - 1945-4589

VL - 9

SP - 1055

EP - 1068

JO - Aging

JF - Aging

IS - 3

ER -

An epigenetic aging clock for dogs and wolves

Abstract

All Science Journal Classification (ASJC) codes

Keywords

Access to Document

Other files and links

Fingerprint

Cite this