An Engineered Methionyl-tRNA Synthetase Enables Azidonorleucine Incorporation in Methionine Prototrophic Bacteria

Diya M. Abdeljabbar; Thomas J. Klein; A. James Link

doi:10.1002/cbic.201100089

An Engineered Methionyl-tRNA Synthetase Enables Azidonorleucine Incorporation in Methionine Prototrophic Bacteria

Diya M. Abdeljabbar, Thomas J. Klein, A. James Link

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Have you Met this? Methionine prototrophic E. coli harboring a single genomic copy of an engineered methionyl-tRNA synthetase (MetRS) gene, metG*, are more efficient at incorporating azidonorleucine (ANL) into recombinant proteins than prototrophic E. coli containing a multicopy plasmid expressing metG* and a genomic copy of the native MetRS (metG).

Original language	English (US)
Pages (from-to)	1699-1702
Number of pages	4
Journal	ChemBioChem
Volume	12
Issue number	11
DOIs	https://doi.org/10.1002/cbic.201100089
State	Published - Jul 25 2011

All Science Journal Classification (ASJC) codes

Molecular Medicine
Molecular Biology
Biochemistry
Organic Chemistry

Keywords

Amino acids
Biotechnology
Genetic code
Genetic engineering
Proteins

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10.1002/cbic.201100089

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abstract = "Have you Met this? Methionine prototrophic E. coli harboring a single genomic copy of an engineered methionyl-tRNA synthetase (MetRS) gene, metG*, are more efficient at incorporating azidonorleucine (ANL) into recombinant proteins than prototrophic E. coli containing a multicopy plasmid expressing metG* and a genomic copy of the native MetRS (metG).",

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AU - Link, A. James

PY - 2011/7/25

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AB - Have you Met this? Methionine prototrophic E. coli harboring a single genomic copy of an engineered methionyl-tRNA synthetase (MetRS) gene, metG*, are more efficient at incorporating azidonorleucine (ANL) into recombinant proteins than prototrophic E. coli containing a multicopy plasmid expressing metG* and a genomic copy of the native MetRS (metG).

KW - Amino acids

KW - Biotechnology

KW - Genetic code

KW - Genetic engineering

KW - Proteins

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An Engineered Methionyl-tRNA Synthetase Enables Azidonorleucine Incorporation in Methionine Prototrophic Bacteria

Abstract

All Science Journal Classification (ASJC) codes

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