An Engineered Methionyl-tRNA Synthetase Enables Azidonorleucine Incorporation in Methionine Prototrophic Bacteria

Diya M. Abdeljabbar, Thomas J. Klein, Aaron James Link

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Have you Met this? Methionine prototrophic E. coli harboring a single genomic copy of an engineered methionyl-tRNA synthetase (MetRS) gene, metG*, are more efficient at incorporating azidonorleucine (ANL) into recombinant proteins than prototrophic E. coli containing a multicopy plasmid expressing metG* and a genomic copy of the native MetRS (metG).

Original languageEnglish (US)
Pages (from-to)1699-1702
Number of pages4
JournalChemBioChem
Volume12
Issue number11
DOIs
StatePublished - Jul 25 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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