An Easy-to-Fabricate Cell Stretcher Reveals Density-Dependent Mechanical Regulation of Collective Cell Movements in Epithelia

Kevin C. Hart, Joo Yong Sim, Matthew A. Hopcroft, Daniel J. Cohen, Jiongyi Tan, W. James Nelson, Beth L. Pruitt

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Mechanical forces regulate many facets of cell and tissue biology. Studying the effects of forces on cells requires real-time observations of single- and multi-cell dynamics in tissue models during controlled external mechanical input. Many of the existing devices used to conduct these studies are costly and complicated to fabricate, which reduces the availability of these devices to many laboratories. Methods: We show how to fabricate a simple, low-cost, uniaxial stretching device, with readily available materials and instruments that is compatible with high-resolution time-lapse microscopy of adherent cell monolayers. In addition, we show how to construct a pressure controller that induces a repeatable degree of stretch in monolayers, as well as a custom MATLAB code to quantify individual cell strains. Results: As an application note using this device, we show that uniaxial stretch slows down cellular movements in a mammalian epithelial monolayer in a cell density-dependent manner. We demonstrate that the effect on cell movement involves the relocalization of myosin downstream of Rho-associated protein kinase (ROCK). Conclusions: This mechanical device provides a platform for broader involvement of engineers and biologists in this important area of cell and tissue biology. We used this device to demonstrate the mechanical regulation of collective cell movements in epithelia.

Original languageEnglish (US)
Pages (from-to)569-581
Number of pages13
JournalCellular and Molecular Bioengineering
Volume14
Issue number6
DOIs
StatePublished - Dec 2021

All Science Journal Classification (ASJC) codes

  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • Cell strain
  • Cellular biomechanics
  • Epithelial monolayer
  • Live-cell imaging
  • Mechanobiology

Fingerprint

Dive into the research topics of 'An Easy-to-Fabricate Cell Stretcher Reveals Density-Dependent Mechanical Regulation of Collective Cell Movements in Epithelia'. Together they form a unique fingerprint.

Cite this