The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes BamHI, HpaI, PvuII, XbaI and XhoI were used to construct a map of the φEC genome.
All Science Journal Classification (ASJC) codes
- Restriction endonuclease mapping
- electron microscopy
- electron microscopy of DNA
- kilobase pairs
- replicative form