An analysis of the genome of actinophage φEC

George H. Brownell; Lynn W. Enquist; Katherine Denniston-Thompson

doi:10.1016/0378-1119(80)90114-6

An analysis of the genome of actinophage φEC

George H. Brownell, Lynn W. Enquist, Katherine Denniston-Thompson

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes BamHI, HpaI, PvuII, XbaI and XhoI were used to construct a map of the φEC genome.

Original language	English (US)
Pages (from-to)	311-314
Number of pages	4
Journal	Gene
Volume	12
Issue number	3-4
DOIs	https://doi.org/10.1016/0378-1119(80)90114-6
State	Published - 1980

All Science Journal Classification (ASJC) codes

Genetics

Keywords

EM
Nocardia
RF
Restriction endonuclease mapping
Rhodococcus
electron microscopy
electron microscopy of DNA
kb
kilobase pairs
replicative form

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10.1016/0378-1119(80)90114-6

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title = "An analysis of the genome of actinophage φEC",

abstract = "The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes BamHI, HpaI, PvuII, XbaI and XhoI were used to construct a map of the φEC genome.",

keywords = "EM, Nocardia, RF, Restriction endonuclease mapping, Rhodococcus, electron microscopy, electron microscopy of DNA, kb, kilobase pairs, replicative form",

author = "Brownell, {George H.} and Enquist, {Lynn W.} and Katherine Denniston-Thompson",

note = "Funding Information: We gratefully acknowledge the technical assistance of Margery Sullivan (LMB; NICHD, NIH) and Mary J. Madden, as well as the encouragement of Dr. George Vande Woude. The senior author was on an assignment agreement with the National Cancer Institute and the work was supported by the Laboratory of Molecular Virology, NCI and a Biomedical Research Grant 5.S07-RR05-365-17.",

year = "1980",

doi = "10.1016/0378-1119(80)90114-6",

language = "English (US)",

volume = "12",

pages = "311--314",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "3-4",

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T1 - An analysis of the genome of actinophage φEC

AU - Brownell, George H.

AU - Enquist, Lynn W.

AU - Denniston-Thompson, Katherine

N1 - Funding Information: We gratefully acknowledge the technical assistance of Margery Sullivan (LMB; NICHD, NIH) and Mary J. Madden, as well as the encouragement of Dr. George Vande Woude. The senior author was on an assignment agreement with the National Cancer Institute and the work was supported by the Laboratory of Molecular Virology, NCI and a Biomedical Research Grant 5.S07-RR05-365-17.

PY - 1980

Y1 - 1980

N2 - The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes BamHI, HpaI, PvuII, XbaI and XhoI were used to construct a map of the φEC genome.

AB - The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes BamHI, HpaI, PvuII, XbaI and XhoI were used to construct a map of the φEC genome.

KW - EM

KW - Nocardia

KW - RF

KW - Restriction endonuclease mapping

KW - Rhodococcus

KW - electron microscopy

KW - electron microscopy of DNA

KW - kb

KW - kilobase pairs

KW - replicative form

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SN - 0378-1119

VL - 12

SP - 311

EP - 314

JO - Gene

JF - Gene

IS - 3-4

ER -

An analysis of the genome of actinophage φEC

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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