Alignment and calibration of a focal neurotransmitter uncaging system

Dmitry V. Sarkisov, Samuel S.H. Wang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Photolysis of caged compounds is a powerful tool for studying subcellular physiological functions. Here we describe protocols for the alignment and calibration of a focal uncaging system. We also report procedures for convenient quantitative calibration of uncaging. Using these methods, we can achieve submicron lateral resolution of photolysis and probe biological function in spines, the smallest signaling compartments of neurons. Initially, the entire alignment procedure takes 4-6 h to perform; periodic fine-tuning of the system takes 1-2 h.

Original languageEnglish (US)
Pages (from-to)828-832
Number of pages5
JournalNature Protocols
Issue number2
StatePublished - Jul 2006

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology


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