Alignment and calibration of a focal neurotransmitter uncaging system

Dmitry V. Sarkisov, Samuel Sheng-Hung Wang

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Photolysis of caged compounds is a powerful tool for studying subcellular physiological functions. Here we describe protocols for the alignment and calibration of a focal uncaging system. We also report procedures for convenient quantitative calibration of uncaging. Using these methods, we can achieve submicron lateral resolution of photolysis and probe biological function in spines, the smallest signaling compartments of neurons. Initially, the entire alignment procedure takes 4-6 h to perform; periodic fine-tuning of the system takes 1-2 h.

Original languageEnglish (US)
Pages (from-to)828-832
Number of pages5
JournalNature Protocols
Volume1
Issue number2
DOIs
StatePublished - Jul 1 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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