Algal p-coumaric acid induces oxidative stress and siderophore biosynthesis in the bacterial symbiont Phaeobacter inhibens

Rurun Wang, Étienne Gallant, Maxwell Z. Wilson, Yihan Wu, Anran Li, Zemer Gitai, Mohammad R. Seyedsayamdost

Research output: Contribution to journalArticlepeer-review

Abstract

The marine alpha-proteobacterium Phaeobacter inhibens engages in intermittent symbioses with microalgae. The symbiosis is biphasic and concludes in a parasitic phase, during which the bacteria release algaecidal metabolites in response to algal p-coumaric acid (pCA). The cell-wide effects of pCA on P. inhibens remain unknown. Herein, we report a microarray-based transcriptomic study and find that genes related to the oxidative stress response and secondary metabolism are upregulated most, while those associated with energy production and motility are downregulated in the presence of pCA. Among genes upregulated is a previously unannotated biosynthetic gene cluster and, using a combination of gene deletions and metabolic profiling, we show that it gives rise to an unreported siderophore, roseobactin. The simultaneous production of algaecides and roseobactin in the parasitic phase allows the bacteria to take up any iron that is released from dying algal cells, thereby securing a limited micronutrient.

Original languageEnglish (US)
Pages (from-to)670-679.e5
JournalCell Chemical Biology
Volume29
Issue number4
DOIs
StatePublished - Apr 21 2022

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Keywords

  • Roseobacter
  • biosynthesis
  • microbial symbiosis
  • natural product
  • oxidative stress response
  • siderophore
  • transcriptomics

Fingerprint

Dive into the research topics of 'Algal p-coumaric acid induces oxidative stress and siderophore biosynthesis in the bacterial symbiont Phaeobacter inhibens'. Together they form a unique fingerprint.

Cite this