@article{5746cf6efc184ac6818eb0d265855e7d,
title = "Advances and challenges in studying hepatitis C virus in its native environment",
abstract = "Approximately 2% of the worldwide population is infected with hepatitis C virus (HCV), the major causative agent of non-A, non-B hepatitis. Although substantial progress has been made in developing tools to dissect the viral life cycle, most in vitro studies rely on hepatoma cell lines, which are functionally disparate from the natural in vivo target of the virus - hepatocytes. To gain insights into virus-host interactions, there is a need for HCV-model systems that more closely mimic the physiological environment of the liver. Here, we discuss recent advances in culture and detection systems that facilitate the study of HCV in primary cells. Use of these new models may help bridge the gap between in vitro studies and clinical research.",
keywords = "cell culture systems, drug development, hepatitis C virus, hepatotropic infections, liver, primary hepatocytes",
author = "Timothy Sheahan and Jones, {Christopher T.} and Alexander Ploss",
note = "Funding Information: This work was funded in part by the Greenberg Medical Institute, National Institutes of Health through the NIH Roadmap for Medical Research, Grant 1 R01 DK085713-01. Information on this Roadmap Transformative R01 Program can be found at http://grants.nih.gov/grants/guide/rfa-files/ RFA-RM-08-029.htm. Timothy Sheahan and Christopher T Jones are recipients of Ruth L Kirschstein National Research Service Awards (F32AI084448 to Timothy Sheahan) from the National Institute of Allergy and Infectious Diseases and National Institute of Health (F32DK081193 to Christopher T Jones). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Allergy and Infectious Diseases or the National Institutes of Health. Alexander Ploss and Christopher T Jones are co-inventors on a patent covering the use of MPCC technology for analyzing human hepatotropic infections. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.",
year = "2010",
month = oct,
doi = "10.1586/egh.10.53",
language = "English (US)",
volume = "4",
pages = "541--550",
journal = "Expert Review of Gastroenterology and Hepatology",
issn = "1747-4124",
publisher = "Expert Reviews Ltd.",
number = "5",
}