Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 α subunit subsequent to infection

R. J. Schneider, B. Safer, S. M. Munemitsu, C. E. Samuel, T. Shenk

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

The virus-associated VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of mRNAs late after infection. Deletion mutant dl331 fails to produce this RNA and, as a result, grows poorly. Three lines of evidence suggest that VAI RNA facilitates translation by preventing inactivation of the function of eukaryotic initiation factor 2 (eIF-2). First, the mutant's translational defect can be relieved by addition of eIF-2 or eIF-2B (GTP recycling factor). Second, extracts of mutant-infected cells exhibit enhanced protein P1/eIF-2α subunit kinase activity. Third, dl331 can grow with nearly normal kinetics in cells that do not express the kinase.

Original languageEnglish (US)
Pages (from-to)4321-4325
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number13
DOIs
StatePublished - 1985

All Science Journal Classification (ASJC) codes

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