TY - JOUR
T1 - Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 α subunit subsequent to infection
AU - Schneider, R. J.
AU - Safer, B.
AU - Munemitsu, S. M.
AU - Samuel, C. E.
AU - Shenk, T.
PY - 1985
Y1 - 1985
N2 - The virus-associated VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of mRNAs late after infection. Deletion mutant dl331 fails to produce this RNA and, as a result, grows poorly. Three lines of evidence suggest that VAI RNA facilitates translation by preventing inactivation of the function of eukaryotic initiation factor 2 (eIF-2). First, the mutant's translational defect can be relieved by addition of eIF-2 or eIF-2B (GTP recycling factor). Second, extracts of mutant-infected cells exhibit enhanced protein P1/eIF-2α subunit kinase activity. Third, dl331 can grow with nearly normal kinetics in cells that do not express the kinase.
AB - The virus-associated VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of mRNAs late after infection. Deletion mutant dl331 fails to produce this RNA and, as a result, grows poorly. Three lines of evidence suggest that VAI RNA facilitates translation by preventing inactivation of the function of eukaryotic initiation factor 2 (eIF-2). First, the mutant's translational defect can be relieved by addition of eIF-2 or eIF-2B (GTP recycling factor). Second, extracts of mutant-infected cells exhibit enhanced protein P1/eIF-2α subunit kinase activity. Third, dl331 can grow with nearly normal kinetics in cells that do not express the kinase.
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U2 - 10.1073/pnas.82.13.4321
DO - 10.1073/pnas.82.13.4321
M3 - Article
C2 - 2989814
AN - SCOPUS:0022416367
SN - 0027-8424
VL - 82
SP - 4321
EP - 4325
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 13
ER -