TY - JOUR
T1 - Adenovirus VAI RNA facilitates the initiation of translation in virus-infected cells
AU - Schneider, Robert J.
AU - Weinberger, Cary
AU - Shenk, Thomas
N1 - Funding Information:
We thank Marilyn Kozak, Brian Safer, and Aaron Shatkin for helpful discussion and suggestions. This work was supported by a grant from the Amerrcan Cancer Society (MV45). R. J. S. is a postdoctoral fellow of the National Cancer Institute (CA07227).
PY - 1984/5
Y1 - 1984/5
N2 - The adenovirus VAI RNA is a small polymerase III-transcribed species that is required for optimal translation of mRNAs late after infection. Mutant d1331 fails to produce this RNA species and, as a result, grows poorly. Mutant-infected cells contain normal levels of late mRNAs, but reduced levels of polypeptides are synthesized late after infection. Translational elongation occurs at normal rates in mutant, as compared to wild-type, virus-infected cells. Initiation of translation occurs with reduced efficiency in d1331-infected cells. VAI RNA is required for formation of a stable 48S preinitiation complex and very likely functions to facilitate the interaction between 43S preinitiation complex and mRNA to form the 48S species.
AB - The adenovirus VAI RNA is a small polymerase III-transcribed species that is required for optimal translation of mRNAs late after infection. Mutant d1331 fails to produce this RNA species and, as a result, grows poorly. Mutant-infected cells contain normal levels of late mRNAs, but reduced levels of polypeptides are synthesized late after infection. Translational elongation occurs at normal rates in mutant, as compared to wild-type, virus-infected cells. Initiation of translation occurs with reduced efficiency in d1331-infected cells. VAI RNA is required for formation of a stable 48S preinitiation complex and very likely functions to facilitate the interaction between 43S preinitiation complex and mRNA to form the 48S species.
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U2 - 10.1016/0092-8674(84)90325-8
DO - 10.1016/0092-8674(84)90325-8
M3 - Article
C2 - 6722874
AN - SCOPUS:0021154949
SN - 0092-8674
VL - 37
SP - 291
EP - 298
JO - Cell
JF - Cell
IS - 1
ER -