Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2α kinase

Jan Kitajewski; Robert J. Schneider; Brian Safer; Susan M. Munemitsu; Charles E. Samuel; Bayar Thimmappaya; Thomas Shenk

doi:10.1016/0092-8674(86)90383-1

Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2α kinase

Jan Kitajewski
, Robert J. Schneider
, Brian Safer
, Susan M. Munemitsu
, Charles E. Samuel
, Bayar Thimmappaya
, Thomas Shenk

Molecular Biology

Research output: Contribution to journal › Article › peer-review

299 Scopus citations

Abstract

The VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of host cell and viral mRNAs late after infection. The growth of a viral mutant that is unable to produce the RNA is inhibited by interferon, while wild-type virus is not affected. VAI RNA prevents activation of the interferon-induced P1 eIF-2α kinase. This inhibition can be reproduced in extracts of interferon-treated cells where purified VAI RNA prevents activation of latent kinase by double-stranded RNA.

Original language	English (US)
Pages (from-to)	195-200
Number of pages	6
Journal	Cell
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(86)90383-1
State	Published - Apr 25 1986

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(86)90383-1

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@article{7f094e092de54c82bf6a1258f648563e,

title = "Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2α kinase",

abstract = "The VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of host cell and viral mRNAs late after infection. The growth of a viral mutant that is unable to produce the RNA is inhibited by interferon, while wild-type virus is not affected. VAI RNA prevents activation of the interferon-induced P1 eIF-2α kinase. This inhibition can be reproduced in extracts of interferon-treated cells where purified VAI RNA prevents activation of latent kinase by double-stranded RNA.",

author = "Jan Kitajewski and Schneider, \{Robert J.\} and Brian Safer and Munemitsu, \{Susan M.\} and Samuel, \{Charles E.\} and Bayar Thimmappaya and Thomas Shenk",

note = "Funding Information: We thank S. Pestka (Roche Institute of Molecular Biology) for a gift of human aA-interferon. This work was supported by a grant from the American Cancer Society (MV-45) to T. Shenk and grants from the National Institutes of Health to C. E. Samuel (Al 12520 and Al 20811) and B. Thimmappaya (Al 18029). J. Kitajewski is a predoctoral trainee of the National institute of General Medical Sciences (GM 07388), R. J. Schneider was a postdoctoral fellow of the National Cancer Institute (CAO7227), 8. Thimmappaya is an Established Investigator of the American Heart Association, and T. Shenk is an American Cancer Society Research Professor.",

year = "1986",

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doi = "10.1016/0092-8674(86)90383-1",

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T1 - Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2α kinase

AU - Kitajewski, Jan

AU - Schneider, Robert J.

AU - Safer, Brian

AU - Munemitsu, Susan M.

AU - Samuel, Charles E.

AU - Thimmappaya, Bayar

AU - Shenk, Thomas

N1 - Funding Information: We thank S. Pestka (Roche Institute of Molecular Biology) for a gift of human aA-interferon. This work was supported by a grant from the American Cancer Society (MV-45) to T. Shenk and grants from the National Institutes of Health to C. E. Samuel (Al 12520 and Al 20811) and B. Thimmappaya (Al 18029). J. Kitajewski is a predoctoral trainee of the National institute of General Medical Sciences (GM 07388), R. J. Schneider was a postdoctoral fellow of the National Cancer Institute (CAO7227), 8. Thimmappaya is an Established Investigator of the American Heart Association, and T. Shenk is an American Cancer Society Research Professor.

PY - 1986/4/25

Y1 - 1986/4/25

N2 - The VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of host cell and viral mRNAs late after infection. The growth of a viral mutant that is unable to produce the RNA is inhibited by interferon, while wild-type virus is not affected. VAI RNA prevents activation of the interferon-induced P1 eIF-2α kinase. This inhibition can be reproduced in extracts of interferon-treated cells where purified VAI RNA prevents activation of latent kinase by double-stranded RNA.

AB - The VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of host cell and viral mRNAs late after infection. The growth of a viral mutant that is unable to produce the RNA is inhibited by interferon, while wild-type virus is not affected. VAI RNA prevents activation of the interferon-induced P1 eIF-2α kinase. This inhibition can be reproduced in extracts of interferon-treated cells where purified VAI RNA prevents activation of latent kinase by double-stranded RNA.

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Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2α kinase

Abstract

All Science Journal Classification (ASJC) codes

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