Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection

J. Logan, T. Shenk

Research output: Contribution to journalArticle

120 Scopus citations

Abstract

A series of adenovirus type 5 variants was constructed to probe the function of the tripartite leader sequence, a 200-nucleotide, 5' noncoding sequence carried on the majority of late viral mRNAs. Recombinant plasmids were constructed that carried the major late transcriptional control region followed by portions of the tripartite leader sequence fused to the E1A coding region. These modified E1A genes were then rebuilt into intact viral chromosomes, replacing the corresponding wild-type region. The leader segments had no effect on the translation of E1A mRNAs early after infection, but the tripartite leader significantly enhanced (5-fold) the efficiency with which the mRNAs were translated after infection.

Original languageEnglish (US)
Pages (from-to)3655-3659
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number12 I
DOIs
StatePublished - 1984

All Science Journal Classification (ASJC) codes

  • General

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