Adenovirus E1B 55-M(r) polypeptide facilitates timely cytoplasmic accumulation of adeno-associated virus mRNAs

Adenovirus provides helper functions that facilitate replication of adeno-associated virus (AAV). Both the adenovirus E1B 55-M(r) and E4 34-M(r) polypeptides are required for efficient and timely accumulation of AAV mRNA, proteins, and DNA. The E1B 55-M(r) polypeptide is also required for rescue of the integrated AAV genome in Detroit 6-D5 cells in a normal time frame. All of these effects probably result from a single, primary delay in AAV mRNA accumulation. The AAV helper function provided by the E1B 55-M(r) and E4 34-M(r) polypeptides appears to closely parallel their normal role in the adenovirus replication cycle.