Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

Clifford P. Brangwynne, Timothy J. Mitchison, Anthony A. Hyman

Research output: Contribution to journalArticle

441 Scopus citations

Abstract

For most intracellular structures with larger than molecular dimensions, little is known about the connection between underlying molecular activities and higher order organization such as size and shape. Here, we show that both the size and shape of the amphibian oocyte nucleolus ultimately arise because nucleoli behave as liquid-like droplets of RNA and protein, exhibiting characteristic viscous fluid dynamics even on timescales of <1 min. We use these dynamics to determine an apparent nucleolar viscosity, and we show that this viscosity is ATP-dependent, suggesting a role for active processes in fluidizing internal contents. Nucleolar surface tension and fluidity cause their restructuring into spherical droplets upon imposed mechanical deformations. Nucleoli exhibit a broad distribution of sizes with a characteristic power law, which we show is a consequence of spontaneous coalescence events. These results have implications for the function of nucleoli in ribosome subunit processing and provide a physical link between activity within a macromolecular assembly and its physical properties on larger length scales.

Original languageEnglish (US)
Pages (from-to)4334-4339
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number11
DOIs
StatePublished - Mar 15 2011

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Active fluids
  • Cytoskeleton
  • Germinal vesicle
  • Nuclear bodies

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