Activation of protein splicing with light in yeast

Amy B. Tyszkiewicz, Tom W. Muir

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

Spatiotemporal regulation of protein function is a key feature of living systems; experimental tools that provide such control are of great utility. Here we report a genetically encoded system for controlling a post-translational process, protein splicing, with light. Studies in Saccharomyces cerevisiae demonstrate that fusion of a photodimerization system from Arabidopsis thaliana to an artificially split intein permits rapid activation of protein splicing to yield a new protein product.

Original languageEnglish (US)
Pages (from-to)303-305
Number of pages3
JournalNature Methods
Volume5
Issue number4
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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