Activation of protein splicing by protease- or light-triggered O to N acyl migration

Miquel Vila-Perelló, Yuichiro Hori, Marc Ribó, Tom W. Muir

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Protein splicing under control: Introduction of an O-acyl isomer of a peptide bond into the naturally split Ssp DnaE intein prevents protein trans splicing, which can be recovered upon triggering an O to N acyl shift by deprotection with protease or light (see scheme). This system allows the splicing of a bacterial toxin to be controlled in response to protease activity.

Original languageEnglish (US)
Pages (from-to)7764-7767
Number of pages4
JournalAngewandte Chemie - International Edition
Volume47
Issue number40
DOIs
StatePublished - Sep 22 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Keywords

  • Acylation
  • Antimicrobial peptides
  • Protein engineering
  • Protein modifications

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