Activation of protein splicing by protease- or light-triggered O to N acyl migration

Miquel Vila-Perelló; Yuichiro Hori; Marc Ribó; Tom W. Muir

doi:10.1002/anie.200802502

Activation of protein splicing by protease- or light-triggered O to N acyl migration

Miquel Vila-Perelló, Yuichiro Hori, Marc Ribó, Tom W. Muir

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Protein splicing under control: Introduction of an O-acyl isomer of a peptide bond into the naturally split Ssp DnaE intein prevents protein trans splicing, which can be recovered upon triggering an O to N acyl shift by deprotection with protease or light (see scheme). This system allows the splicing of a bacterial toxin to be controlled in response to protease activity.

Original language	English (US)
Pages (from-to)	7764-7767
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	47
Issue number	40
DOIs	https://doi.org/10.1002/anie.200802502
State	Published - Sep 22 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
Catalysis

Keywords

Acylation
Antimicrobial peptides
Protein engineering
Protein modifications

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10.1002/anie.200802502

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abstract = "Protein splicing under control: Introduction of an O-acyl isomer of a peptide bond into the naturally split Ssp DnaE intein prevents protein trans splicing, which can be recovered upon triggering an O to N acyl shift by deprotection with protease or light (see scheme). This system allows the splicing of a bacterial toxin to be controlled in response to protease activity.",

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AU - Vila-Perelló, Miquel

AU - Hori, Yuichiro

AU - Ribó, Marc

AU - Muir, Tom W.

PY - 2008/9/22

Y1 - 2008/9/22

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AB - Protein splicing under control: Introduction of an O-acyl isomer of a peptide bond into the naturally split Ssp DnaE intein prevents protein trans splicing, which can be recovered upon triggering an O to N acyl shift by deprotection with protease or light (see scheme). This system allows the splicing of a bacterial toxin to be controlled in response to protease activity.

KW - Acylation

KW - Antimicrobial peptides

KW - Protein engineering

KW - Protein modifications

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ER -

Activation of protein splicing by protease- or light-triggered O to N acyl migration

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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