Activation of mouse retrovirus by herpes simplex virus type 1 cloned DNA fragments

Ann L. Boyd; Lynn Enquist; George F. Vand Woude; Berge Hampar

doi:10.1016/0042-6822(80)90140-3

Activation of mouse retrovirus by herpes simplex virus type 1 cloned DNA fragments

Ann L. Boyd, Lynn Enquist, George F. Vand Woude, Berge Hampar

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Abstract

Cloned DNA fragments from herpes simplex virus (HSV) type 1 (strain Patton) were tested for activation of endogenous mouse retrovirus in BALB/3T3 cells. Activation within the L region of HSV-1 DNA was observed with the ∼3.4-kilobase pair (kbp) BamHI fragment which contains the virus thymidine kinase (TK) gene, and the ∼5.3-kbp EcoRI L fragment. Activation by the TK-containing BamHI fragment was abrogated by digestion with EcoRI. Activation within the S region of HSV-1 DNA was observed with the ∼15.2-kbp EcoRI H ragment and the ∼8.4-kbp Eco RI/HindIII H/G fragment. Assaying for retrovirus activation serves as an additional parameter for mapping biological functions within the HSV genome.

Original language	English (US)
Pages (from-to)	228-231
Number of pages	4
Journal	Virology
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(80)90140-3
State	Published - May 1980

All Science Journal Classification (ASJC) codes

Virology

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10.1016/0042-6822(80)90140-3

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title = "Activation of mouse retrovirus by herpes simplex virus type 1 cloned DNA fragments",

abstract = "Cloned DNA fragments from herpes simplex virus (HSV) type 1 (strain Patton) were tested for activation of endogenous mouse retrovirus in BALB/3T3 cells. Activation within the L region of HSV-1 DNA was observed with the ∼3.4-kilobase pair (kbp) BamHI fragment which contains the virus thymidine kinase (TK) gene, and the ∼5.3-kbp EcoRI L fragment. Activation by the TK-containing BamHI fragment was abrogated by digestion with EcoRI. Activation within the S region of HSV-1 DNA was observed with the ∼15.2-kbp EcoRI H ragment and the ∼8.4-kbp Eco RI/HindIII H/G fragment. Assaying for retrovirus activation serves as an additional parameter for mapping biological functions within the HSV genome.",

author = "Boyd, {Ann L.} and Lynn Enquist and {Vand Woude}, {George F.} and Berge Hampar",

note = "Funding Information: This research was supported in part by Contract NOl-CO-75380 with the National Cancer Institute, NIH, Bethesda, Maryland 20205.",

year = "1980",

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doi = "10.1016/0042-6822(80)90140-3",

language = "English (US)",

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pages = "228--231",

journal = "Virology",

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T1 - Activation of mouse retrovirus by herpes simplex virus type 1 cloned DNA fragments

AU - Boyd, Ann L.

AU - Enquist, Lynn

AU - Vand Woude, George F.

AU - Hampar, Berge

N1 - Funding Information: This research was supported in part by Contract NOl-CO-75380 with the National Cancer Institute, NIH, Bethesda, Maryland 20205.

PY - 1980/5

Y1 - 1980/5

N2 - Cloned DNA fragments from herpes simplex virus (HSV) type 1 (strain Patton) were tested for activation of endogenous mouse retrovirus in BALB/3T3 cells. Activation within the L region of HSV-1 DNA was observed with the ∼3.4-kilobase pair (kbp) BamHI fragment which contains the virus thymidine kinase (TK) gene, and the ∼5.3-kbp EcoRI L fragment. Activation by the TK-containing BamHI fragment was abrogated by digestion with EcoRI. Activation within the S region of HSV-1 DNA was observed with the ∼15.2-kbp EcoRI H ragment and the ∼8.4-kbp Eco RI/HindIII H/G fragment. Assaying for retrovirus activation serves as an additional parameter for mapping biological functions within the HSV genome.

AB - Cloned DNA fragments from herpes simplex virus (HSV) type 1 (strain Patton) were tested for activation of endogenous mouse retrovirus in BALB/3T3 cells. Activation within the L region of HSV-1 DNA was observed with the ∼3.4-kilobase pair (kbp) BamHI fragment which contains the virus thymidine kinase (TK) gene, and the ∼5.3-kbp EcoRI L fragment. Activation by the TK-containing BamHI fragment was abrogated by digestion with EcoRI. Activation within the S region of HSV-1 DNA was observed with the ∼15.2-kbp EcoRI H ragment and the ∼8.4-kbp Eco RI/HindIII H/G fragment. Assaying for retrovirus activation serves as an additional parameter for mapping biological functions within the HSV genome.

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Activation of mouse retrovirus by herpes simplex virus type 1 cloned DNA fragments

Abstract

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