Activation of an autoregulated protein kinase by conditional protein splicing

Henning D. Mootz; Elyse S. Blum; Tom W. Muir

doi:10.1002/anie.200460941

Activation of an autoregulated protein kinase by conditional protein splicing

Henning D. Mootz, Elyse S. Blum, Tom W. Muir

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

A protein rheostat: A strategy for controlling enzyme activity by cleavage of a low-affinity "pseudosubstrate" inhibitor from an autoregulated version of the enzyme (see figure) is described. Peptide-bond cleavage was mediated by conditional protein splicing. The small molecule rapamycin was used to induce intein trans-splicing of the catalytic subunit of cAMP-dependent protein kinase A (PKA).

Original language	English (US)
Pages (from-to)	5189-5192
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	43
Issue number	39
DOIs	https://doi.org/10.1002/anie.200460941
State	Published - Oct 4 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
Catalysis

Keywords

Enzymes
Inhibitors
Protein kinase
Protein splicing

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10.1002/anie.200460941

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year = "2004",

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AU - Blum, Elyse S.

AU - Muir, Tom W.

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AB - A protein rheostat: A strategy for controlling enzyme activity by cleavage of a low-affinity "pseudosubstrate" inhibitor from an autoregulated version of the enzyme (see figure) is described. Peptide-bond cleavage was mediated by conditional protein splicing. The small molecule rapamycin was used to induce intein trans-splicing of the catalytic subunit of cAMP-dependent protein kinase A (PKA).

KW - Enzymes

KW - Inhibitors

KW - Protein kinase

KW - Protein splicing

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SN - 1433-7851

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 39

ER -

Activation of an autoregulated protein kinase by conditional protein splicing

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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