A protein rheostat: A strategy for controlling enzyme activity by cleavage of a low-affinity "pseudosubstrate" inhibitor from an autoregulated version of the enzyme (see figure) is described. Peptide-bond cleavage was mediated by conditional protein splicing. The small molecule rapamycin was used to induce intein trans-splicing of the catalytic subunit of cAMP-dependent protein kinase A (PKA).
All Science Journal Classification (ASJC) codes
- Protein kinase
- Protein splicing