Activation of an autoregulated protein kinase by conditional protein splicing

Henning D. Mootz, Elyse S. Blum, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


A protein rheostat: A strategy for controlling enzyme activity by cleavage of a low-affinity "pseudosubstrate" inhibitor from an autoregulated version of the enzyme (see figure) is described. Peptide-bond cleavage was mediated by conditional protein splicing. The small molecule rapamycin was used to induce intein trans-splicing of the catalytic subunit of cAMP-dependent protein kinase A (PKA).

Original languageEnglish (US)
Pages (from-to)5189-5192
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number39
StatePublished - Oct 4 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Catalysis


  • Enzymes
  • Inhibitors
  • Protein kinase
  • Protein splicing


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