Activation of an autoregulated protein kinase by conditional protein splicing

Henning D. Mootz, Elyse S. Blum, Thomas William Muir

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

A protein rheostat: A strategy for controlling enzyme activity by cleavage of a low-affinity "pseudosubstrate" inhibitor from an autoregulated version of the enzyme (see figure) is described. Peptide-bond cleavage was mediated by conditional protein splicing. The small molecule rapamycin was used to induce intein trans-splicing of the catalytic subunit of cAMP-dependent protein kinase A (PKA).

Original languageEnglish (US)
Pages (from-to)5189-5192
Number of pages4
JournalAngewandte Chemie - International Edition
Volume43
Issue number39
DOIs
StatePublished - Oct 4 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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