Activation and Sorting of Human White Blood Cells

Robert H. Carlson, Chris Gabel, Shirley Chan, Robert H. Austin

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We demonstrate a novel activation behavior of human leucocyte adhesion under physiological flow conditions in a microfabricated silicon array of channels with length scales similar to those of human capillaries. Vital nuclei stains and cell specific, flourochrome labeled antibodies reveal that the equilibrium distribution of stuck cells in the arrays displays a strong dependence on cell type and nuclear morphology, and there is eventual separation of the two cell types in the array. The distortion of the cells is the same as they experience in vivo and the response of the granulocytes is consistent with a model describing adhesion as a function of the distortion of the cell by its environment; in other words, activated adhesion. We propose that this complex non-random behavior is due to a deformation activated change in the cells relevant to observed in vivo behavior.

Original languageEnglish (US)
Pages (from-to)39-47
Number of pages9
JournalBiomedical Microdevices
Volume1
Issue number1
DOIs
StatePublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Keywords

  • Activation
  • Array
  • Blood
  • Microfabrication
  • Sorting
  • White blood cells

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