Actin-like cytoskeleton filaments contribute to cell mechanics in bacteria

Siyuan Wang, Hugo Arellano-Santoyo, Peter A. Combs, Joshua W. Shaevitz

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

A filamentous cytoskeleton largely governs the physical shape and mechanical properties of eukaryotic cells. In bacteria, proteins homologous to all three classes of eukaryotic cytoskeletal filaments have recently been discovered. These proteins are essential for the maintenance of bacterial cell shape and have been shown to guide the localization of key cell-wall-modifying enzymes. However, whether the bacterial cytoskeleton is stiff enough to affect the overall mechanical rigidity of a cell has not been probed. Here, we used an optical trap to measure the bending rigidity of live Escherichia coli cells. We find that the actin-homolog MreB contributes nearly as much to the stiffness of a cell as the peptidoglycan cell wall. By quantitatively modeling these measurements, our data indicate that the MreB is rigidly linked to the cell wall, increasing the mechanical stiffness of the overall system. These data are the first evidence that the bacterial cytoskeleton contributes to the mechanical integrity of a cell in much the same way as it does in eukaryotes.

Original languageEnglish (US)
Pages (from-to)9182-9185
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number20
DOIs
StatePublished - May 18 2010

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Escherichia coli
  • MreB
  • Optical trapping

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