Acousto-optical deflector-based patterned ultraviolet uncaging of neurotransmitter for the study of neuronal integration

Eugene F. Civillico; Shy Shoham; Daniel H. O'Connor; Dmitry V. Sarkisov; Samuel S.H. Wang

doi:10.1101/pdb.top070631

Acousto-optical deflector-based patterned ultraviolet uncaging of neurotransmitter for the study of neuronal integration

Eugene F. Civillico, Shy Shoham, Daniel H. O'Connor, Dmitry V. Sarkisov, Samuel S.H. Wang

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice of photosensitive molecules depends on the research question and will influence the design of the experimental apparatus. This article describes an acousto-optical deflector (AOD)-based system for rapid ultraviolet (UV) photolysis in arbitrary spatial and temporal patterns. Some basics of caged neurotransmitters and the theory of operation of AODs are covered, as are descriptions for implementing the system.

Original language	English (US)
Pages (from-to)	867-872
Number of pages	6
Journal	Cold Spring Harbor Protocols
Volume	7
Issue number	8
DOIs	https://doi.org/10.1101/pdb.top070631
State	Published - Aug 2012

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1101/pdb.top070631

Cite this

@article{b0c9aaf674b44c71a63685577ef8777b,

title = "Acousto-optical deflector-based patterned ultraviolet uncaging of neurotransmitter for the study of neuronal integration",

abstract = "The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice of photosensitive molecules depends on the research question and will influence the design of the experimental apparatus. This article describes an acousto-optical deflector (AOD)-based system for rapid ultraviolet (UV) photolysis in arbitrary spatial and temporal patterns. Some basics of caged neurotransmitters and the theory of operation of AODs are covered, as are descriptions for implementing the system.",

author = "Civillico, {Eugene F.} and Shy Shoham and O'Connor, {Daniel H.} and Sarkisov, {Dmitry V.} and Wang, {Samuel S.H.}",

year = "2012",

month = aug,

doi = "10.1101/pdb.top070631",

language = "English (US)",

volume = "7",

pages = "867--872",

journal = "Cold Spring Harbor Protocols",

issn = "1940-3402",

publisher = "Cold Spring Harbor Laboratory Press",

number = "8",

}

TY - JOUR

T1 - Acousto-optical deflector-based patterned ultraviolet uncaging of neurotransmitter for the study of neuronal integration

AU - Civillico, Eugene F.

AU - Shoham, Shy

AU - O'Connor, Daniel H.

AU - Sarkisov, Dmitry V.

AU - Wang, Samuel S.H.

PY - 2012/8

Y1 - 2012/8

N2 - The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice of photosensitive molecules depends on the research question and will influence the design of the experimental apparatus. This article describes an acousto-optical deflector (AOD)-based system for rapid ultraviolet (UV) photolysis in arbitrary spatial and temporal patterns. Some basics of caged neurotransmitters and the theory of operation of AODs are covered, as are descriptions for implementing the system.

AB - The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice of photosensitive molecules depends on the research question and will influence the design of the experimental apparatus. This article describes an acousto-optical deflector (AOD)-based system for rapid ultraviolet (UV) photolysis in arbitrary spatial and temporal patterns. Some basics of caged neurotransmitters and the theory of operation of AODs are covered, as are descriptions for implementing the system.

UR - http://www.scopus.com/inward/record.url?scp=84865649524&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865649524&partnerID=8YFLogxK

U2 - 10.1101/pdb.top070631

DO - 10.1101/pdb.top070631

M3 - Article

C2 - 22854573

AN - SCOPUS:84865649524

SN - 1940-3402

VL - 7

SP - 867

EP - 872

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 8

ER -

Acousto-optical deflector-based patterned ultraviolet uncaging of neurotransmitter for the study of neuronal integration

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this