Acetyl phosphate and the activation of two-component response regulators

William R. McCleary, Jeffry B. Stock

Research output: Contribution to journalArticle

258 Scopus citations

Abstract

Several bacterial response regulator proteins (CheY, NR(I), PhoB, and OmpR) become phosphorylated in vitro when incubated with acetyl phosphate. In the presence of high levels of acetyl phosphate and Mg2+, CheY reached steady state phosphorylation in less than 30 s; NR(I) and PhoB reached steady state more slowly (t 1/2 ) to steady state of 1.5 and >15 min, respectively). A simple method was developed to measure acetyl phosphate levels in Escherichia coli grown in defined media. Levels of acetyl phosphate were elevated in cells grown in pyruvate, glucose, and glucuronic acid and were low in cells grown in fructose, glycerol, and fumarate. The effects of varying the intracellular amounts of acetyl phosphate on chemotaxis and the osmo-response were also investigated. Acetyl phosphate was not required but did influence each of these responses. These results suggest that acetyl phosphate may influence either the sensitivity or the magnitude of an adaptive response.

Original languageEnglish (US)
Pages (from-to)31567-31572
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number50
StatePublished - Dec 16 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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