Accurate proteome-wide protein quantification from high-resolution 15N mass spectra

Zia Khan, Sasan Amini, Joshua S. Bloom, Cristian Ruse, Amy A. Caudy, Leonid Kruglyak, Mona Singh, David H. Perlman, Saeed Tavazoie

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

In quantitative mass spectrometry-based proteomics, the metabolic incorporation of a single source of 15N-labeled nitrogen has many advantages over using stable isotope-labeled amino acids. However, the lack of a robust computational framework for analyzing the resulting spectra has impeded wide use of this approach. We have addressed this challenge by introducing a new computational methodology for analyzing 15N spectra in which quantification is integrated with identification. Application of this method to an Escherichia coli growth transition reveals significant improvement in quantification accuracy over previous methods.

Original languageEnglish (US)
Article numberR122
JournalGenome biology
Volume12
Issue number12
DOIs
StatePublished - Dec 19 2011

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

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