TY - JOUR
T1 - A Vibrio cholerae autoinducer-receptor pair that controls biofilm formation
AU - Papenfort, Kai
AU - Silpe, Justin E.
AU - Schramma, Kelsey R.
AU - Cong, Jian Ping
AU - Seyedsayamdost, Mohammad R.
AU - Bassler, Bonnie Lynn
N1 - Funding Information:
This work was supported by the Howard Hughes Medical Institute, NIH Grant 2R37GM065859, and National Science Foundation Grant MCB-0948112 (to B.L.B.). K.P. was supported by a career development award from the Human Frontiers Science Program (CDA00024/2016-C) and DFG Grant PA2820/1. J.E.S. was supported by the Department of Defense (DoD) through the National Defense Science and Engineering Graduate Fellowship (NDSEG) program. M.R.S. gratefully acknowledges support from the Pew Biomedical Scholars Program. K.R.S. was supported by the Eli Lilly-Edward C. Taylor Fellowship in Chemistry. We thank the members of the Bassler lab for helpful discussions. We especially thank M. Donia, T. Srikumar, I. Pelczer, and S. Kyin for help in initial analyses of DPO.
Publisher Copyright:
© The Author(s) 2017.
PY - 2017/5/1
Y1 - 2017/5/1
N2 - Quorum sensing (QS) is a cell-cell communication process that enables bacteria to track cell population density and orchestrate collective behaviors. QS relies on the production and detection of, and the response to, extracellular signal molecules called autoinducers. In Vibrio cholerae, multiple QS circuits control pathogenesis and biofilm formation. Here, we identify and characterize a new QS autoinducer-receptor pair. The autoinducer is 3,5-dimethylpyrazin-2-ol (DPO). DPO is made from threonine and alanine, and its synthesis depends on threonine dehydrogenase (Tdh). DPO binds to and activates a transcription factor, VqmA. The VqmA-DPO complex activates expression of vqmR, which encodes a small regulatory RNA. VqmR represses genes required for biofilm formation and toxin production. We propose that DPO allows V. cholerae to regulate collective behaviors to, among other possible roles, diversify its QS output during colonization of the human host.
AB - Quorum sensing (QS) is a cell-cell communication process that enables bacteria to track cell population density and orchestrate collective behaviors. QS relies on the production and detection of, and the response to, extracellular signal molecules called autoinducers. In Vibrio cholerae, multiple QS circuits control pathogenesis and biofilm formation. Here, we identify and characterize a new QS autoinducer-receptor pair. The autoinducer is 3,5-dimethylpyrazin-2-ol (DPO). DPO is made from threonine and alanine, and its synthesis depends on threonine dehydrogenase (Tdh). DPO binds to and activates a transcription factor, VqmA. The VqmA-DPO complex activates expression of vqmR, which encodes a small regulatory RNA. VqmR represses genes required for biofilm formation and toxin production. We propose that DPO allows V. cholerae to regulate collective behaviors to, among other possible roles, diversify its QS output during colonization of the human host.
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U2 - 10.1038/nchembio.2336
DO - 10.1038/nchembio.2336
M3 - Article
C2 - 28319101
AN - SCOPUS:85015672491
SN - 1552-4450
VL - 13
SP - 551
EP - 557
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 5
ER -