A viable simian virus 40 variant that carries a newly generated sequence reiteration in place of the normal duplicated enhancer element

C. Swimmer, T. Shenk

Research output: Contribution to journalArticle

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Abstract

A segment comprising the transcriptional enhancer elements was deleted from a recombinant plasmid carrying the simian virus 40 genome. The mutated viral chromosome was excised from the plasmid and propagated through several cycles of growth in monkey kidney cells. A variant was obtained that carried reiterations of sequences that span both sides of the deleted enhancer region. The mutant virus, dup1495, displays a lag in its growth kinetics as compared to its parent, but it ultimately generates wild-type yields. The mutant virus expresses early mRNAs at near-normal levels, and the reiterated sequence functioned in cis to enhance transformation of mouse cells by the herpes virus thymidine kinase gene. Thus dup1495 reiterated segments encode enhancer activity even though their primary sequence is radically different from that of the normal simian virus 40 enhancer.

Original languageEnglish (US)
Pages (from-to)6652-6656
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number21 I
DOIs
StatePublished - 1984
Externally publishedYes

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