TY - JOUR
T1 - A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins
AU - Wilhelm, Vivian
AU - Miquel, Alvaro
AU - Burzio, Luis O.
AU - Rosemblatt, Mario
AU - Engel, Esteban
AU - Valenzuela, Sebastián
AU - Parada, Gustavo
AU - Valenzuela, Pablo D.T.
N1 - Funding Information:
This work was support in part by CORFO FDI and grants DI 23-04 (to M.R.) and DI 22-04 (to P.V.) from the University Andrés Bello. We thank Drs. Bernardita Méndez and David Holmes for critical reading and helpful suggestions on the manuscript.
PY - 2006/6/5
Y1 - 2006/6/5
N2 - We report here the protective effect against piscirickettsiosis elicited in fish by a mixture of recombinant proteins. A comparative genomics strategy was used on a genomic library of Piscirickettsia salmonis in order to select optimal candidates for a recombinant subunit vaccine to protect fish from rickettsial septicaemia (SRS). Based on this information, 15 P. salmonis ORFs encoding heat shock proteins, virulence factors, membrane bound and other surface exposed antigens, were isolated and expressed. Seven of the most promising antigens were formulated in three mixtures (V1-V3) containing two or three recombinant proteins each and injected into salmon to test their protective efficacy. Two of the three formulations (V1, V2) elicited a strong protective response in a challenge against the pathogen, which was coincident with the humoral response against the corresponding recombinant proteins present in each formulation. V1, formulated with recombinant chaperonines Hsp60, Hsp70 and flagellar protein FlgG of P. salmonis achieved the highest level of protection with a relative percent survival (RPS) of 95%.
AB - We report here the protective effect against piscirickettsiosis elicited in fish by a mixture of recombinant proteins. A comparative genomics strategy was used on a genomic library of Piscirickettsia salmonis in order to select optimal candidates for a recombinant subunit vaccine to protect fish from rickettsial septicaemia (SRS). Based on this information, 15 P. salmonis ORFs encoding heat shock proteins, virulence factors, membrane bound and other surface exposed antigens, were isolated and expressed. Seven of the most promising antigens were formulated in three mixtures (V1-V3) containing two or three recombinant proteins each and injected into salmon to test their protective efficacy. Two of the three formulations (V1, V2) elicited a strong protective response in a challenge against the pathogen, which was coincident with the humoral response against the corresponding recombinant proteins present in each formulation. V1, formulated with recombinant chaperonines Hsp60, Hsp70 and flagellar protein FlgG of P. salmonis achieved the highest level of protection with a relative percent survival (RPS) of 95%.
KW - Piscirickettsia salmonis
KW - Recombinant antigens
KW - Recombinant proteins
KW - Subunit vaccine
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U2 - 10.1016/j.vaccine.2006.03.027
DO - 10.1016/j.vaccine.2006.03.027
M3 - Article
C2 - 16697089
AN - SCOPUS:33744469119
SN - 0264-410X
VL - 24
SP - 5083
EP - 5091
JO - Vaccine
JF - Vaccine
IS - 23
ER -