A general method is described for creating chimeric proteins by transposition of subdomain-sized gene fragments. The method uses three sequential PCR steps to circumvent several limitations inherent in transposition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method permits flexibility in the choice of host molecule and insertion site.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Chimeric proteins
- Protein structure
- Protein subdomains