A three-step PCR protocol for construction of chimeric proteins

Rita Grandori, Kara Struck, Kira Giovanielli, Jannette Carey

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A general method is described for creating chimeric proteins by transposition of subdomain-sized gene fragments. The method uses three sequential PCR steps to circumvent several limitations inherent in transposition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method permits flexibility in the choice of host molecule and insertion site.

Original languageEnglish (US)
Pages (from-to)1099-1100
Number of pages2
JournalProtein Engineering
Volume10
Issue number9
DOIs
StatePublished - 1997

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Keywords

  • Chimeric proteins
  • PCR
  • Protein structure
  • Protein subdomains
  • WrbA

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