A three-step PCR protocol for construction of chimeric proteins

Rita Grandori, Kara Struck, Kira Giovanielli, Jannette Carey

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


A general method is described for creating chimeric proteins by transposition of subdomain-sized gene fragments. The method uses three sequential PCR steps to circumvent several limitations inherent in transposition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method permits flexibility in the choice of host molecule and insertion site.

Original languageEnglish (US)
Pages (from-to)1099-1100
Number of pages2
JournalProtein Engineering
Issue number9
StatePublished - 1997

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry


  • Chimeric proteins
  • PCR
  • Protein structure
  • Protein subdomains
  • WrbA


Dive into the research topics of 'A three-step PCR protocol for construction of chimeric proteins'. Together they form a unique fingerprint.

Cite this