A three-step PCR protocol for construction of chimeric proteins

Rita Grandori; Kara Struck; Kira Giovanielli; Jannette Carey

doi:10.1093/protein/10.9.1099

A three-step PCR protocol for construction of chimeric proteins

Rita Grandori, Kara Struck, Kira Giovanielli, Jannette Carey

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A general method is described for creating chimeric proteins by transposition of subdomain-sized gene fragments. The method uses three sequential PCR steps to circumvent several limitations inherent in transposition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method permits flexibility in the choice of host molecule and insertion site.

Original language	English (US)
Pages (from-to)	1099-1100
Number of pages	2
Journal	Protein Engineering
Volume	10
Issue number	9
DOIs	https://doi.org/10.1093/protein/10.9.1099
State	Published - 1997

All Science Journal Classification (ASJC) codes

Molecular Biology
Biochemistry

Keywords

Chimeric proteins
PCR
Protein structure
Protein subdomains
WrbA

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10.1093/protein/10.9.1099

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abstract = "A general method is described for creating chimeric proteins by transposition of subdomain-sized gene fragments. The method uses three sequential PCR steps to circumvent several limitations inherent in transposition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method permits flexibility in the choice of host molecule and insertion site.",

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author = "Rita Grandori and Kara Struck and Kira Giovanielli and Jannette Carey",

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AU - Grandori, Rita

AU - Struck, Kara

AU - Giovanielli, Kira

AU - Carey, Jannette

PY - 1997

Y1 - 1997

N2 - A general method is described for creating chimeric proteins by transposition of subdomain-sized gene fragments. The method uses three sequential PCR steps to circumvent several limitations inherent in transposition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method permits flexibility in the choice of host molecule and insertion site.

AB - A general method is described for creating chimeric proteins by transposition of subdomain-sized gene fragments. The method uses three sequential PCR steps to circumvent several limitations inherent in transposition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method permits flexibility in the choice of host molecule and insertion site.

KW - Chimeric proteins

KW - PCR

KW - Protein structure

KW - Protein subdomains

KW - WrbA

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ER -

A three-step PCR protocol for construction of chimeric proteins

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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