A TATA-like sequence located downstream of the transcription initiation site is required for expression of an RNA polymerase II transcribed gene

J. Carcamo, E. Maldonado, P. Cortes, M. H. Ahn, Ilho-Ha, Y. Kasai, J. Flint, D. Reinberg

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

TFIID, the TATA-binding protein, was found to stimulate transcription from the adenovirus IVa2 promoter, a promoter considered to lack the TATA motif. Remarkably, a TATA-like sequence element located downstream of the transcription start site binds TFIID and is required for TFIID-dependent transcription from the IVa2 promoter. Transcription from the IVa2 and the adjacent adenovirus major late promoter (Ad-MLP) is divergent, and the cap sites are separated by 212 nucleotides. Nevertheless, the TATA motifs of the IVa2 promoter and Ad-MLP were found to be oriented in the same direction. An initiator motif around the transcription start site is located in the IVa2 promoter, and in contrast to the TATA motifs, the IVa2-initiator is in the opposite orientation with respect to the initiator of the Ad-MLP. A model is presented in which the polar nature of the initiator governs the direction of transcription. We propose that RNA polymerase II and accessory factors recognize the initiator in an orientation-dependent fashion. The recognition of the IVa2 initiator by RNA polymerase is enhanced by the binding of TFIID to the downstream TATA motif.

Original languageEnglish (US)
Pages (from-to)1611-1622
Number of pages12
JournalGenes and Development
Volume4
Issue number9
DOIs
StatePublished - Jan 1 1990

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • Ad-MLP
  • RNA polymerase II
  • TATA motif
  • TFIID

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