A suppressor of cell death caused by the loss of σE downregulates extracytoplasmic stress responses and outer membrane vesicle production in Escherichia coli

Julie E. Button, Thomas J. Silhavy, Natividad Ruiz

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

When envelope biogenesis is compromised or damage to envelope components occurs, bacteria trigger signaling cascades, which lead to the production of proteins that combat snch extracytoplasmic stresses. In Escherichia coli, there are three pathways known to deal with envelope stresses: the Bae, Cpx, and σE responses. Although the effectors of the Bae and Cpx responses are not essential in E. coli, the effector of the σE response, the sigma factor RpoE (σE), is essential for viability. However, mutations that suppress the lethality of an rpoE-null allele can be easily obtained, and here we describe how we have isolated at least four classes of these suppressors. We present the first description of one such suppressor class, loss-of-function mutations in ydcQ, a gene encoding a putative DNA-binding protein. In wild-type rpoE+ strains, ydcQ mutants have two distinct phenotypes: extracytoplasmic stress responses are significantly downregulated, and the production of outer membrane vesicles is severely reduced. We present a model in which σE is not essential per se but, rather, we propose that rpoE mutant cells die, possibly because they overreact to the absence of this σ factor by triggering a cell death signal.

Original languageEnglish (US)
Pages (from-to)1523-1530
Number of pages8
JournalJournal of bacteriology
Volume189
Issue number5
DOIs
StatePublished - Mar 2007

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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