@article{8f32246eefd747bbb6b26b6fe4c8a11c,
title = "A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry",
abstract = "Modern proteomics requires reagents for exact quantification of peptides in complex mixtures. Peptide labelling is most typically achieved with isobaric tags that consist of a balancer and a reporter part that separate in the gas phase. An ingenious distribution of stable isotopes provides multiple reagents with identical molecular weight but a different mass of the reporter groups, allowing relative quantification of multiple samples in one measurement. Here we report a new isobaric labelling reagent, where the balancer and the reporter are linked by a sulfoxide group, which, based on the sulfoxide pyrolysis, leads to easy and asymmetric cleavage at low fragmentation energy. The fragmentation of our new design is significantly improved, yielding more intense complementary ion signals, allowing complementary ion cluster analysis as well.",
keywords = "complementary ion clusters, isobaric labelling, mass spectrometry, peptides, proteomics, sulfoxide pyrolysis",
author = "Michael Stadlmeier and Jana Bogena and Miriam Wallner and Martin W{\"u}hr and Thomas Carell",
note = "Funding Information: We thank the Deutsche Forschungsgemeinschaft for financial support (SPP1784, CA275 and GRK2062) and the Bundesministerium f{\"u}r Bildung und Forschung (BMBF) for additional support by way of the Excellence Cluster CiPS M. Further support from the European Union through the Marie Curie International Training and Mobility Network “Clickgene” (grant No. 642023) is acknowledged. M. W{\"u}hr thanks the U.S. Department of Energy for support (DOE grant DE-SC0018260). M.S. thanks the Fonds der Chemischen Industrie for a predoctoral fellowship. Funding Information: We thank the Deutsche Forschungsgemeinschaft for financial support (SPP1784, CA275 and GRK2062) and the Bundes-ministerium f{\"u}r Bildung und Forschung (BMBF) for additional support by way of the Excellence Cluster CiPSM. Further support from the European Union through the Marie Curie International Training and Mobility Network “Click-gene” (grant No. 642023) is acknowledged. M. W{\"u}hr thanks the U.S. Department of Energy for support (DOE grant DESC0018260). M.S. thanks the Fonds der Chemischen Industrie for a predoctoral fellowship. Publisher Copyright: {\textcopyright} 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2018",
month = mar,
day = "5",
doi = "10.1002/anie.201708867",
language = "English (US)",
volume = "57",
pages = "2958--2962",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "11",
}