A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry

Michael Stadlmeier, Jana Bogena, Miriam Wallner, Martin Wühr, Thomas Carell

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Modern proteomics requires reagents for exact quantification of peptides in complex mixtures. Peptide labelling is most typically achieved with isobaric tags that consist of a balancer and a reporter part that separate in the gas phase. An ingenious distribution of stable isotopes provides multiple reagents with identical molecular weight but a different mass of the reporter groups, allowing relative quantification of multiple samples in one measurement. Here we report a new isobaric labelling reagent, where the balancer and the reporter are linked by a sulfoxide group, which, based on the sulfoxide pyrolysis, leads to easy and asymmetric cleavage at low fragmentation energy. The fragmentation of our new design is significantly improved, yielding more intense complementary ion signals, allowing complementary ion cluster analysis as well.

Original languageEnglish (US)
Pages (from-to)2958-2962
Number of pages5
JournalAngewandte Chemie - International Edition
Volume57
Issue number11
DOIs
StatePublished - Mar 5 2018

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Keywords

  • complementary ion clusters
  • isobaric labelling
  • mass spectrometry
  • peptides
  • proteomics
  • sulfoxide pyrolysis

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