A size threshold governs Caenorhabditis elegans Developmental Progression

Sravanti Uppaluri, Clifford P. Brangwynne

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


The growth of organisms from humans to bacteria is affected by environmental conditions. However, mechanisms governing growth and size control are not well understood, particularly in the context of changes in food availability in developing multicellular organisms. Here, we use a novel microfluidic platform to study the impact of diet on the growth and development of the nematode Caenorhabditis elegans. This device allows us to observe individual worms throughout larval development, quantify their growth as well as pinpoint the moulting transitions marking successive developmental stages. Under conditions of low food availability, worms grow very slowly, but do not moult until they have achieved a threshold size. The time spent in larval stages can be extended by over an order of magnitude, in agreement with a simple threshold size model. Thus, a critical worm size appears to trigger developmental progression, and may contribute to prolonged lifespan under dietary restriction.

Original languageEnglish (US)
Article number20151283
JournalProceedings of the Royal Society B: Biological Sciences
Issue number1813
StatePublished - Aug 19 2015

All Science Journal Classification (ASJC) codes

  • General Immunology and Microbiology
  • General Environmental Science
  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


  • Caenorhabditis elegans
  • Development
  • Growth
  • Moult
  • Size


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