@article{28b81ac9ac0c4b36bcef282c01c6f6f6,
title = "A size-invariant bud-duration timer enables robustness in yeast cell size control",
abstract = "Cell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant {\textquoteleft}timer{\textquoteright} specifying the duration of S/ G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis.",
author = "Allard, {Corey A.H.} and Franziska Decker and Weiner, {Orion D.} and Toettcher, {Jared E.} and Graziano, {Brian R.}",
note = "Funding Information: Support for this work was provided by a Post-Physiology Course Award from the Marine Biological Laboratory to C.A.H.A., the Thomas B. Grave and Elizabeth F. Grave Scholarship to F.D., and the National Institutes of Health [GM118167 to O.D.W., DP2EB024247 to J.E.T., and T32HL773125 to B.R.G.]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There was no additional external funding received for this study. We thank Anna Payne-Tobin Jost for helpful discussions, Alba Diz-Mu{\~n}oz for experimental assistance, Fred Chang for critical reading of the manuscript and Nairi Hartooni for reagents. We also thank the organizers of the 2015 MBL Physiology course, Wallace Marshall, Jennifer Lippincott-Schwartz, and Rob Phillips, as well as all the course staff for creating the well-run, intellectually-stimulating environment from which this project grew. Publisher Copyright: {\textcopyright} 2018 Allard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2018",
month = dec,
doi = "10.1371/journal.pone.0209301",
language = "English (US)",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",
}