A single rat fibronectin gene generates three different mRNAs by alternative splicing of a complex exon

J. W. Tamkun, J. E. Schwarzbauer, R. O. Hynes

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

Three fibronectin mRNAs exist in rat liver, differing by the presence or absence of segments of 285 or 360 bases at a point within the coding region. We previously proposed that the three mRNAs are encoded by a single gene and arise via alternative splicing of a common transcript. In order to test this hypothesis, we have isolated clones spanning approximately half of the fibronectin gene from a Fisher rat genomic library; blot hybridization analyses reveal the presence of only one fibronectin gene in the haploid rat genome. We determined the sequence of a portion (1221 nucleotides) of this gene. This sequence shows clearly that the three fibronectin mRNAs encoded by this gene are generated by a pattern of alternative splicing in which one 5' splice site can be paired with any one of three 3' splice sites, one at the beginning of, and two within, a single complex exon.

Original languageEnglish (US)
Pages (from-to)5140-5144
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number16 I
DOIs
StatePublished - 1984
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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