TY - GEN
T1 - A simplified model for lysogenic regulation through DNA looping
AU - Anderson, L. Meadow
AU - Yang, Haw
PY - 2008
Y1 - 2008
N2 - The lysogenic state of bacteriophage lambda has been a key model for understanding gene regulation. A single protein, CI repressor, maintains this state by blocking gene expression from two promoters separated by ̃2.3 kbp of DNA. CI controls its own expression by positive and negative feedback by binding to Or to regulate the Prm promoter. Not only does CI interact directly with operator DNA, but CI tetramers bound to Ol and Or can form an octamer, looping the DNA that lies between them. Previous studies show that Ol can assist with negative regulation of Prm, and we recently showed that DNA looping can also enhance looping activation. In this paper we present a new interpretation of our recent experimental data based a new crystal structure of CI. The simpler model suggested by the new structural information predicts that the most common forms of the DNA loop have similar activation behavior, and are enhanced ̃2.2-fold relative to unlooped activation.
AB - The lysogenic state of bacteriophage lambda has been a key model for understanding gene regulation. A single protein, CI repressor, maintains this state by blocking gene expression from two promoters separated by ̃2.3 kbp of DNA. CI controls its own expression by positive and negative feedback by binding to Or to regulate the Prm promoter. Not only does CI interact directly with operator DNA, but CI tetramers bound to Ol and Or can form an octamer, looping the DNA that lies between them. Previous studies show that Ol can assist with negative regulation of Prm, and we recently showed that DNA looping can also enhance looping activation. In this paper we present a new interpretation of our recent experimental data based a new crystal structure of CI. The simpler model suggested by the new structural information predicts that the most common forms of the DNA loop have similar activation behavior, and are enhanced ̃2.2-fold relative to unlooped activation.
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U2 - 10.1109/iembs.2008.4649226
DO - 10.1109/iembs.2008.4649226
M3 - Conference contribution
C2 - 19162729
AN - SCOPUS:61849129469
SN - 9781424418152
T3 - Proceedings of the 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08 - "Personalized Healthcare through Technology"
SP - 607
EP - 610
BT - Proceedings of the 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08
PB - IEEE Computer Society
T2 - 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08
Y2 - 20 August 2008 through 25 August 2008
ER -