A simplified miRNA-based gene silencing method for Drosophila melanogaster

Benjamin Haley, David Hendrix, Vinh Trang, Michael Levine

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

MicroRNA-based RNA interference is commonly used to produce loss-of-function phenotypes in mammalian systems, but is used only sparingly in invertebrates such as Caenorhabditis elegans and Drosophila melanogaster. Here, we evaluate this method in transgenic strains of D. melanogaster and cultured S2 cells. High throughput-ready expression vectors were developed that permit rapid cloning of synthetic hairpin RNAs. As proof of concept, this method was used for the efficient silencing of dpp gene activity in the adult wing, and the analysis of the general RNA Polymerase II (Pol II) elongation factor, Nelf-E.

Original languageEnglish (US)
Pages (from-to)482-490
Number of pages9
JournalDevelopmental biology
Volume321
Issue number2
DOIs
StatePublished - Sep 15 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Keywords

  • Decapentaplegic
  • Drosophila
  • Method
  • Nelf
  • RNAi
  • Transgenic
  • miRNA
  • shRNA
  • siRNA

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