A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm

Felipe Moreno; Audree V. Fowler; Michael Hall; Thomas J. Silhavy; Irving Zabin; Maxime Schwartz

doi:10.1038/286356a0

A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm

Felipe Moreno, Audree V. Fowler, Michael Hall, Thomas J. Silhavy, Irving Zabin, Maxime Schwartz

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Abstract

Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme β-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a β-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.

Original language	English (US)
Pages (from-to)	356-359
Number of pages	4
Journal	Nature
Volume	286
Issue number	5771
DOIs	https://doi.org/10.1038/286356a0
State	Published - 1980
Externally published	Yes

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title = "A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm",

abstract = "Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme β-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a β-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.",

author = "Felipe Moreno and Fowler, {Audree V.} and Michael Hall and Silhavy, {Thomas J.} and Irving Zabin and Maxime Schwartz",

year = "1980",

doi = "10.1038/286356a0",

language = "English (US)",

volume = "286",

pages = "356--359",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "5771",

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T1 - A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm

AU - Moreno, Felipe

AU - Fowler, Audree V.

AU - Hall, Michael

AU - Silhavy, Thomas J.

AU - Zabin, Irving

AU - Schwartz, Maxime

PY - 1980

Y1 - 1980

N2 - Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme β-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a β-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.

AB - Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme β-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a β-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.

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U2 - 10.1038/286356a0

DO - 10.1038/286356a0

M3 - Article

C2 - 6772962

AN - SCOPUS:0018937774

SN - 0028-0836

VL - 286

SP - 356

EP - 359

JO - Nature

JF - Nature

IS - 5771

ER -

A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm

Abstract

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