A selective fluorescent sensor for detecting lead in living cells

Qiwen He; Evan W. Miller; Audrey P. Wong; Christopher J. Chang

doi:10.1021/ja063029x

A selective fluorescent sensor for detecting lead in living cells

Qiwen He, Evan W. Miller, Audrey P. Wong, Christopher J. Chang

Research output: Contribution to journal › Article › peer-review

349 Scopus citations

Abstract

We present the synthesis, properties, and biological applications of Leadfluor-1 (LF1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively imaging Pb²⁺ in aqueous solution and in living cells. LF1 combines a fluorescein chromophore and a pseudocrown receptor to provide good selectivity for Pb²⁺ over a range of biologically and environmentally relevant metal ions in aqueous solution, with sensitivity to parts per billion EPA limits for allowable lead in drinking water. In addition to these attributes, imaging experiments further show that LF1 is the first small-molecule reagent that can be delivered into living cells and report changes in intracellular Pb²⁺ levels.

Original language	English (US)
Pages (from-to)	9316-9317
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	128
Issue number	29
DOIs	https://doi.org/10.1021/ja063029x
State	Published - Jul 26 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja063029x

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title = "A selective fluorescent sensor for detecting lead in living cells",

abstract = "We present the synthesis, properties, and biological applications of Leadfluor-1 (LF1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively imaging Pb2+ in aqueous solution and in living cells. LF1 combines a fluorescein chromophore and a pseudocrown receptor to provide good selectivity for Pb2+ over a range of biologically and environmentally relevant metal ions in aqueous solution, with sensitivity to parts per billion EPA limits for allowable lead in drinking water. In addition to these attributes, imaging experiments further show that LF1 is the first small-molecule reagent that can be delivered into living cells and report changes in intracellular Pb2+ levels.",

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AB - We present the synthesis, properties, and biological applications of Leadfluor-1 (LF1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively imaging Pb2+ in aqueous solution and in living cells. LF1 combines a fluorescein chromophore and a pseudocrown receptor to provide good selectivity for Pb2+ over a range of biologically and environmentally relevant metal ions in aqueous solution, with sensitivity to parts per billion EPA limits for allowable lead in drinking water. In addition to these attributes, imaging experiments further show that LF1 is the first small-molecule reagent that can be delivered into living cells and report changes in intracellular Pb2+ levels.

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A selective fluorescent sensor for detecting lead in living cells

Abstract

All Science Journal Classification (ASJC) codes

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